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文献和实验Synthesis of Oligodeoxynucleotides with 5′‐Caps Binding RNA Targets
acid cap), 1C95 (tryptophan cap), 1KSE (oxolinic acid cap), and 1X6W (trimethoxystilbene cap). The DNA is shown in gray and the stacking moiety of the caps are shown as orange line drawings with smaller line width
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(1) Remove vitelline membrane from stage 21-23 embryos (n = 300). Collect head tissues (about anterior 1/4 or 1/3 of the embryo) in 1x MBS. (2) Fix the tissues in 0.5x MBS/1% formaldehyde (4-5 ml in a screw cap glass vial) for 15 min
Materials2 ml size Sarstedt screw cap tubesBIO-RAD Disposable Chromatography Column （BIO-RAD cat. #732-6008）Oligo dT celluloseRNase-free water5.0 M NaCl0.5 M EDTA1.0 M Tris, pH 7.410% SDS70% EtOHBefore starting protocol1. Measure out 75 mg of oligo
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