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文献和实验Western blot and gel overlays（蛋白印记与凝胶覆盖）
相关专题 I illustrate western blot s and gel overlayson this page. See Old and Primrose for details. Western blotting: SDS-Page An antibody prepared a known protein is used as a probe. The antibody
Semi-dry Transfer Apparatus Biorad, Cat# 170-3940, or equivalent Power Supply 0 - 100 VDC (adj. current to 1 Amp) Immobilon-Ptransfer membrane 0.45 µm pore size; cut to same size as gel (Millipore, Cat# IPVH304-FO, or equivalent) Filter Paper
General Western Blot Protocol Many variations exist among protocols for running Western blots. These variations address all aspects of the procedure from the choice of gel buffers and membranes to transfer conditions, blocking and processing
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