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文献和实验) Stains‐all (Aldrich) 4‐ and 8‐mL screw‐thread glass vials (Wheaton) 15
Methylation of Fatty Acids (Kropinski Method)脂肪酸甲基化
nmoles/100ml (usually 10mg of C15 in 100ml of reagent.Weigh 10mg of whole cells or 1 mg of LPS into a clean screw-cap tube.Add 1ml of MeOH-HCl reagent/internal standard into each tube and vortex.Heat at 100oC for 20 minutes. (Note: each tube
Methylation of Fatty Acids (Kropinski Method)
of 400 nmoles/100ml (usually 10mg of C15 in 100ml of reagent. Weigh 10mg of whole cells or 1 mg of LPS into a clean screw-cap tube. Add 1ml of MeOH-HCl reagent/internal standard into each tube and vortex. Heat at 100o C for 20 minutes
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