手机验证
—20℃,12个月
2年
20
康朗生物
pGEM-7Zf(+)
5ug
质粒类型: | 大肠杆菌表达载体 |
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载体大小: | 2997 bp (查看载体序列) |
克隆方法: | 多克隆位点,限制性内切酶 |
载体抗性: | Ampicillin (氨苄青霉素) |
产品编号 | 产品名称 | 规格 | 价格 |
---|---|---|---|
KL1887 | pGEM-7Zf(+) | 5ug质粒 |
询价 |
The pGEM®-7Zf(+) and pGEM®-7Zf(–) Vectors are derivatives of the pGEM®-3Zf(+) Vector and contain the origin of replication of the filamentous phage f1. These plasmids serve as standard cloning vectors, as templates for in vitro transcription and can be used for the production of circular ssDNA. These plasmids contain SP6 and T7 RNA polymerase promoters flanking a region of multiple cloning sites within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region is unique and includes restriction sites for ApaI, AatII, SphI, XbaI, XhoI, EcoRI, KpnI, SmaI, ClaI, HindIII, BamHI, SacI, BstXI and NsiI. This arrangement is designed specifically for generating unidirectional deletions with the Erase-a-Base™ System. pGEM®-7Zf(+) and pGEM®-7Zf(–) Vectors are identical except for the orientation of the f1 origin.
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