Restriction digests of the clone give the following sizes (kb): BamHI–6.6; SalI–6.6; PstI–6.6. (ATCC staff) A 3.1 kb EcoRI/DraI fragment of pNM482 was ligated to a 3.5 kb EcoRI/PvuII fragment of pUB110. bleR = bleomycin-pheomycin resistance. (ATCC staff) One of a series (ATCC 37637 – 37639) of 3 plasmid vectors for characterization of regulatory systems in Bacillus subtilis. Inserted genes can be fused to E.coli lacZ, with one vector in the series for each of the 3 translational reading frames. Medium is 1179 V Y medium. Hosts: Bacillus subtilis 168 SB1207, Bacillus, Staphylococcus aureus.