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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
低温运输,-20℃保存
- 保质期:
1年
- 英文名:
pBlueScript II XR
- 库存:
大量
- 供应商:
信裕生物
质粒编号:
质粒名称: pBlueScript II XR
其他名称:
质粒大小(bp): 3000
抗性:
质粒类型: Bacterial Expression
是否病毒: Nonviral
启动子:
稳转或瞬转:
组成型或诱导性:
表达水平:
蛋白标签:
测序引物:
GenBank号:
其他属性: 5' Sequencing 1 Primer: T7 Fwd
Notes: PBlueScript II pre-digested with XhoI/EcoRI
质粒图谱:
质粒序列:
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文献和实验The Queensland Institute for Medical Research (QIMR) lab's method for automated sequencing for expressed sequence tags from Schistosoma japonicum and/or S. mansoni cDNA libraries constructed in the directional vectors lambda ZAP II and UNI ZAP XR
Site-Directed Mutagenesis with LA-PCR Technology
) (pBluescript� II SK(�), KS (�)) and pGEM� vectors (Promega, Madison, WI) (pGEM�-3Zf,4Z) by adding the advantage of LA-PCR technology™ (TaKaRa Shuzo Co., Kyoto, Japan). The protocol uses a combination of a primer designed for introducing a mutation at the target
Cloning PCR products using TA vectors
which can be used for this, including the pBluescript derivative pCR-Script [TM] SK(+) cloning kit from Stratagene, pCR [TM] II supplied with the TA Cloning [TM] kit from Invitrogen, pT7Blue T-Vector kit from Novagen, pGEM[R]-T from Promega, and the SureClone [TM
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