质粒编号: 质粒名称: pBC12BI 其他名称: 质粒大小(bp): 4070 抗性: 质粒类型: Unspecified 是否病毒: 启动子: 稳转或瞬转: 组成型或诱导性: 表达水平: 蛋白标签: 测序引物: GenBank号: 其他属性: cDNA clones without an initiation codon may be cloned in frame into the unique BamHI site 3' to the AUG of the rat preproinsulin gene. This results in 5 – 6 additional amino terminal amino acids from the insulin signal peptide. [1] Genomic sequences are normally inserted between unique HindIII and SmaI sites (5' to 3'). This deletes the preproinsulin gene from the vector. [1] cDNA sequences with a translation initiation codon are usually inserted between the unique HindIII and BamHI sites (5' to 3'). [1] Polyadenylation signals (and possibly carboxy terminal amino acids) are contributed by the preproinsulin gene. [1] This was constructed from pXF3 (pBR322 minus the poison sequence), a SV40 ori, and a rat preproinsulin genomic sequence under the control of RSV LTR (pBC81delta4 cut at BglII and BalI and inserted at the BamHI and ClaI sites). [2] Restriction digests of the clone give the following sizes (kb): BamHI–4.1; EcoRI–2.8,0.78,0.44; HindIII–4.1; BstEII–4.1. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli RR1, vertebrate cells, E.coli, monkey COS-1 cell line, mammal cell line, avian cell line. 质粒图谱: