This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 127-140 of Human p28 ING5protein (Inhibitor of growth family, member 5).
内容
0.02 M Potassium Phosphate 0.15 M Sodium Chloride pH 7.2 0.01% (w/v) Sodium Azide
纯化方式
Immunoaffinity purified
浓度
1.52 mg/mL by UV absorbance at 280 nm
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Western Blot, 1:500 - 1:1, 000, humanDirect ELISA, 1:4, 000 - 1:16, 000, human
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[list_product_images]Western blot analysis of p28 ING5 expression in HeLa cell nuclear extracts. This western blot shows reactivity with purified recombinant TAP tagged human ING5 protein (lane 3) and does not recognize TAP tagged ING4 on the same membrane (lane 2). A mock purification is shown in lane 1. p28 ING5 at 45KD was detected using goat anti-p28 ING5 Antigen Affinity purified polyclonal antibody (Catalog # A04974) at 1:500. Personal communication Jacques Cote.|Western blot analysis of p28 ING5 expression in purified recombinant human ING5 protein (lane 1). p28 ING5 at 36KD was detected using goat anti-p28 ING5 Antigen Affinity purified polyclonal antibody (Catalog # A04974) at 1:1500. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1003).[/list_product_images]