Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
纯化方式
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
产品形态
Liquid
保存条件
At -20°C for one year. At 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.
*最佳稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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Figure 2. Immunohistochemistry validation of EEF2 using Anti-EEF2 (A50) Antibody (A00830).
Immunohistochemistry (IHC) analyzes of EEF2 (A50) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
For more protocol information of IHC|
Figure 1. Western blotting validation for Anti-EEF2 (A50) Antibody A00830
Western blot (WB) analysis of eEF2 (A50) polyclonal antibody at 1:500 dilution
Lane1:HepG2 cell lysate
Lane2:Mouse kidney tissue lysate
Lane3:Rat kidney tissue lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration[/list_product_images]