手机验证
0
0
0
0
单克隆
human
0
5-7天
博士德生物
WB,IHC-P,ELISA
0
200ul
产品名称 | CDK9 Antibody |
---|---|
产品描述 | Anti-CDK9 Antibody validated for IHC, WB, ELISA in Human. |
文献引用格式 | CDK9 Antibody (Boster Biological Technology, Wuhan, China. Catalog #BM3152) |
应用范围 | IHC, WB, ELISA |
抗体来源 | Mouse |
抗体亚型 | IgG1 |
免疫原 | Purified recombinant fragment of human CDK9 expressed in E. Coli. |
抗体规格 | 200ul/1180元 |
克隆 | Monoclonal |
---|---|
克隆号 | 单克隆抗体:IgG1;克隆号:1B5D10 |
产品形态 | Liquid |
内容 | Ascitic fluid containing 0.03% sodium azide. |
储存条件 | 连续使用时4℃存储,保质期六个月;长期存储时建议分装为10ul以上小包装-20℃存储,并避免反复冻融,保质期一年。 |
小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
基因名 | CDK9 |
---|---|
基因全名 | Cyclin-dependent kinase 9 |
蛋白功能 | Ubiquitous. |
Tissue Specificity | Protein kinase involved in the regulation of transcription. Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A, SUPT5H and RDBP. This complex is inactive when in the 7SK snRNP complex form. Phosphorylates EP300, MYOD1, RPB1/POLR2A and AR, and the negative elongation factors DSIF and NELF. Regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors (e.g. TNF-inducible RELA/p65 activation and IL-6-inducible STAT3 signaling). Promotes RNA synthesis in genetic programs for cell growth, differentiation and viral pathogenesis. P-TEFb is also involved in cotranscriptional histone modification, mRNA processing and mRNA export. Modulates a complex network of chromatin modifications including histone H2B monoubiquitination (H2Bub1), H3 lysine 4 trimethylation (H3K4me3) and H3K36me3; integrates phosphorylation during transcription with chromatin modifications to control co-transcriptional histone mRNA processing. The CDK9/cyclin-K complex has also a kinase activity towards CTD of RNAP II and can substitute for CDK9/cyclin-T P-TEFb in vitro. Replication stress response protein; the CDK9/cyclin-K complex is required for genome integrity maintenance, by promoting cell cycle recovery from replication arrest and limiting single- stranded DNA amount in response to replication stress, thus reducing the breakdown of stalled replication forks and avoiding DNA damage. In addition, probable function in DNA repair of isoform 2 via interaction with KU70/XRCC6. Promotes cardiac myocyte enlargement. RPB1/POLR2A phosphorylation on 'Ser-2' in CTD activates transcription. AR phosphorylation modulates AR transcription factor promoter selectivity and cell growth. DSIF and NELF phosphorylation promotes transcription by inhibiting their negative effect. The phosphorylation of MYOD1 enhances its transcriptional activity and thus promotes muscle differentiation. . |
Sequence Similarities | Cyclin-dependent kinase 9;2.7.11.22;2.7.11.23;C-2K;Cell division cycle 2-like protein kinase 4;Cell division protein kinase 9;Serine/threonine-protein kinase PITALRE;Tat-associated kinase complex catalytic subunit;CDK9;CDC2L4, TAK; |
Uniprot ID | CDK9: P50750 |
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. (provided by RefSeq)Tissue specificity: Ubiquitous.
为了提供最优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
---|---|
Western blotting | 1:100-400 |
Immunohistochemistry in paraffin section | 1:50-200 |
Immunocytochemistry in fixed cells | 1:50-200 |
Flow Cytometry | 1:40-80 |
enzyme-linked immuno sorbent assay | 1:2000 |
*最优稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
点击图片放大
[list_product_images]CDK9(BM3152)(MW:43KD)Jurkat,A431,HEK293细胞裂解,免疫印迹分析。|Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues (left) and rectum cancer tissues (right) using CDK9 mouse mAb with DAB staining. |Red: Control Antigen (100ng);Purple: Antigen (10ng);Green: Antigen (50ng);Blue: Antigen (100ng);[/list_product_images]
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。