Core component of nucleosome. Nucleosomes wrap andcompact DNA into chromatin, limiting DNA accessibility to thecellular machineries which require DNA as a template. Histonesthereby play a central role in transcription regulation, DNArepair, DNA replication and chromosomal stability. DNAaccessibility is regulated via a complex set of post-translationalmodifications of histones, also called histone code, andnucleosome remodeling.
Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called €histone code€. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases.
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[list_product_images]To determine the titer of the antibody, an ELISA was performed using a serial dilution of Anti-H3pan polyclonal antibody (Catalog # CI1126), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:400.|Western blot analysis of H3pan expression in histone extracts from NB4 (human promyelocytic leukemia) cells treated with all-trans retinoic acid (ATRA) for 168h (lane 1) and 24h (lane 2), and in untreated control cells (lane 3). H3pan was detected using Anti-H3pan polyclonal antibody (Catalog # CI1126) at 1/1000 dilution.[/list_product_images]