Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. *Blocking peptide 可以联系我们购买。¥300/1ml
免疫原
This antibody is raised in rabbit against mouse Wdr5 (WD (tryptophan-aspartate) repeat domain protein 5), using two KLH-conjugated synthetic peptides containing an amino acid sequence from the central part of the protein.
抗体规格
100μl
产品详情
克隆
Polyclonal
产品形态
Liquid
内容
Whole antiserum from rabbit containing 0.05% azide. *可以提供无载体抗体。
储存条件
At -20℃ for one year, at 4℃ for one month. Avoid repeated freezing and thawing.
Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. Aspart of the MLL1/MLL complex it is involved in methylation anddimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylationrepresents a specific tag for epigenetic transcriptionalactivation. As part of the NSL complex it may be involved inacetylation of nucleosomal histone H4 on several lysine residues.May regulate osteoblasts differentiation..
Wdr5 belongs to the family of WD repeat proteins which are involved in different cellular processes such as cell cycle progression, signal transduction, apoptosis, and gene regulation. It is a component of the Set1A and Set1B histone H3 methylation complexes. These complexes methylate lysine 4 of H3, thereby activating gene transcription. Wdr5 interacts with H3 dimethyl K4, but not with tri or mono methylated H3K4.
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[list_product_images]To determine the titer of the antibody, an ELISA was performed using a serial dilution of Anti- Wdr5 polyclonal antibody (Catalog # CI1102). By plotting the absorbance against the antibody dilution,the titer of the antibody was estimated to be 1:12,300.|Western blot analysis of Wdr5 expression in whole cell lysates from mouse fibroblastst (NIH3T3) (lane 1), whole cell lysates from embryonic stem cells (E14Tg2a) (lane 2). Wdr5 at 36KD was detected using Anti-Wdr5 polyclonal antibody (Catalog # CI1102) at 1/1000 dilution.[/list_product_images]