Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. *Blocking peptide 可以联系我们购买。¥300/1ml
免疫原
This antibody is raised in rabbit against mouse Ehmt1 (euchromatic histone-lysine N-methyltransferase 1), using three KLH-conjugated synthetic peptides containing a sequence from the central part of the protein.
抗体规格
100μl
产品详情
克隆
Polyclonal
产品形态
Liquid
内容
Whole antiserum from rabbit containing 0.05% azide. *可以提供无载体抗体。
储存条件
At -20℃ for one year, at 4℃ for one month. Avoid repeated freezing and thawing.
Histone methyltransferase that specifically mono- anddimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2,respectively) in euchromatin. H3K9me represents a specific tag forepigenetic transcriptional repression by recruiting HP1 proteinsto methylated histones. Also weakly methylates 'Lys-27' of histoneH3 (H3K27me). Also required for DNA methylation, the histonemethyltransferase activity is not required for DNA methylation,suggesting that these 2 activities function independently.Probably targeted to histone H3 by different DNA-binding proteinslike E2F6, MGA, MAX and/or DP1. During G0 phase, it probablycontributes to silencing of MYC- and E2F-responsive genes,suggesting a role in G0/G1 transition in cell cycle. In additionto the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53..
Tissue Specificity
Widely expressed..
Sequence Similarities
Belongs to the class V-like SAM-binding methyltransferase superfamily.
Ehmt1 is a histone methyltransferase which specifically methylates histone H3 on lysine 9. H3K9 methylation represents a marker for epigenetic transcriptional repression. This repression is established by recruiting HP1 proteins to the methylated histone. H3K9 methylation may contribute to silencing of MYC and E2F responsive genes and plays a role in murine embryogenesis.
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[list_product_images]To determine the titer of the antibody, an ELISA was performed using a serial dilution of Anti- Ehmt1 polyclonal antibody (Catalog # CI1107). The plates were coated with a mix of the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:10,600.|Western blot analysis of Ehmt1 expression in nuclear extracts from HeLa cells (40 ?g). Ehmt1 at 138KD was detected using Anti-Ehmt1 polyclonal antibody (Catalog # CI1107) at 1/1000 dilution.[/list_product_images]