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免疫原
This antibody is raised in rabbit against human DNMT3A, (DNA methyltransferase 3A), using a KLH-conjugated synthetic peptide corresponding to amino acids 107-121.
抗体规格
50μg/64μl
产品详情
克隆
Polyclonal
产品形态
Liquid
内容
Affinity purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300. *可以提供无载体抗体。
储存条件
At -20℃ for one year, at 4℃ for one month. Avoid repeated freezing and thawing.
Required for genome-wide de novo methylation and isessential for the establishment of DNA methylation patterns duringdevelopment. DNA methylation is coordinated with methylation ofhistones. It modifies DNA in a non-processive manner and alsomethylates non-CpG sites. May preferentially methylate DNA linkerbetween 2 nucleosomal cores and is inhibited by histone H1. Playsa role in paternal and maternal imprinting. Required formethylation of most imprinted loci in germ cells. Acts as atranscriptional corepressor for ZBTB18. Recruited to trimethylated'Lys-36' of histone H3 (H3K36me3) sites. Can actively represstranscription through the recruitment of HDAC activity..
Tissue Specificity
Highly expressed in fetal tissues, skeletalmuscle, heart, peripheral blood mononuclear cells, kidney, and atlower levels in placenta, brain, liver, colon, spleen, smallintestine and lung..
Sequence Similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.
DNMT3A catalyses the genome wide de novo methylation of CpG residues. DNA methylation on CpG residues by DNMT3A regulates gene expression and is essential for development. DNMT3A is strongly expressed in embryonic stem cells, but low in adult somatic cells. DNA methylation is coordinated with methylation of histones. DNMT3A binds to SETDB1 and HDAC1, and is involved in the repression of transcription from promoters containing an E2F binding site.
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[list_product_images]To determine the titer of the antibody, an ELISA was performed using a serial dilution of Anti-DNMT3A polyclonal antibody (Catalog # CI1063), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:400.|Human embryonic kidney cells (HEK293T) were transiently transfected with an expression vector for GAL4-tagged DNMT3A. Whole cell extracts were immunoprecipitated with 2 ?g of Anti-DNMT3A polyclonal antibody (Catalog # CI1063). The presence of GAL4-DNMT3A in the non-treated cell extracts and in the immunoprecipitates was demonstrated by western blot analysis with anti-GAL4 antibody (lane 1 and 2 respectively). Alternatively, GAL4-DNMT3A was immunoprecipitated and western blot analysis was performed with the DNMT3A antibody at 1/2000 dilution (lane 3).[/list_product_images]