手机验证
0
0
0
0
单克隆
zebrafish,human,drosophila
0
3-4周
博士德生物
WB,ELISA,Dot blot,ChIP-seq,ChIP-qPCR,ChIP
0
50μg/56μl
产品名称 | H3K36me3 Antibody ChIP Grade |
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产品描述 | Rabbit IgG polyclonal antibody for histone H3, trimethylated at lysine 36 (H3K36me3). Tested with ChIP,ChIP-seq,ELISA,Dot blot,WB in Human, Zebrafish, Drosophila. |
文献引用格式 | H3K36me3 Antibody ChIP Grade (Boster Biological Technology, Wuhan, China. Catalog #CI1051) |
应用范围 | WB, ELISA, Dot blot, ChIP-seq, ChIP-qPCR, ChIP |
推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. Boster recommends high sensitivity ChIP-seq Kit (CK1001 & CK1002) for Chromatin Immunoprecipitation. *Blocking peptide 可以联系我们购买。¥300/1ml |
免疫原 | This antibody is raised in rabbit against histone H3, trimethylated at lysine 36 (H3K36me3), using a KLH-conjugated synthetic peptide. |
抗体规格 | 50μg/56μl |
克隆 | Polyclonal |
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产品形态 | Liquid |
内容 | Affinity purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300. *可以提供无载体抗体。 |
储存条件 | At -20℃ for one year, at 4℃ for one month. Avoid repeated freezing and thawing. |
小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
基因名 | HIST1H3A |
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基因全名 | Histone H3.1 |
蛋白功能 | Core component of nucleosome. Nucleosomes wrap andcompact DNA into chromatin, limiting DNA accessibility to thecellular machineries which require DNA as a template. Histonesthereby play a central role in transcription regulation, DNArepair, DNA replication and chromosomal stability. DNAaccessibility is regulated via a complex set of post-translationalmodifications of histones, also called histone code, andnucleosome remodeling. |
Tissue Specificity | |
Sequence Similarities | |
Uniprot ID | HIST1H3A: P68431 |
Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called €histone code€. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Trimethylation of H3K36 is associated with actively transcribed regions.
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[list_product_images]ChIP assays were performed using HeLa cells, Anti-H3K36me3 polyclonal antibody (Catalog # CI1051) and optimized PCR primer sets for qPCR. A titration consisting of 1, 2, 5 and 10μg of antibody per ChIP experiment was analyzed. IgG (2μg/IP) was used as a negative IP control. QPCR was performed with primers for the promoter and coding region of the active GAPDH, for a region located 1 kb upstream of the GAPDH promoter and for the Sat2 satellite repeat.|ChIP was performed with 2 μg of Anti-H3K36me3 polyclonal antibody (Catalog # CI1051) on sheared chromatin from 1 million HeLaS3 cells. IgG (2 μg/IP) was used as a negative IP control. The IP’d DNA was analysed by QPCR with optimized PCR primer pairs for the promoter and coding region of the active GAPDH, for a region located 1 kb upstream of the GAPDH promoter and for the coding region of the active ACTB gene (figure 1A). The IP’d DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 1B shows the obtained profiles in genomic regions of chromosome 12 (including the GAPDH positive control), 7 (including the ACTB positive control), 14 and 3, respectively. These results clearly show an enrichment of the H3K36me3 at active genes.|ChIP was performed with 2 μg of Anti-H3K36me3 polyclonal antibody (Catalog # CI1051) on sheared chromatin from 1 million HeLaS3 cells. IgG (2 μg/IP) was used as a negative IP control. The IP’d DNA was analysed by QPCR with optimized PCR primer pairs for the promoter and coding region of the active GAPDH, for a region located 1 kb upstream of the GAPDH promoter and for the coding region of the active ACTB gene (figure 1A). The IP’d DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 1B shows the obtained profiles in genomic regions of chromosome 12 (including the GAPDH positive control), 7 (including the ACTB positive control), 14 and 3, respectively. These results clearly show an enrichment of the H3K36me3 at active genes.|A Dot Blot analysis was performed to test the cross reactivity of Anti-H3K36me3 polyclonal antibody (Catalog # CI1051) with peptides containing other H3 and H4 modifications and the unmodified sequence. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. This figure shows a high specificity of the antibody for the modification of interest.|To determine the titer of the antibody, an ELISA was performed using a serial dilution of Anti-H3K36me3 polyclonal antibody (Catalog # CI1051) and the crude serum. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:19,300.|Western blot analysis of H3K36me3 expression in histone extracts from HeLa cells (15 μg). H3K36me3 was detected using Anti-H3K36me3 polyclonal antibody (Catalog # CI1051) at 1/1000 dilution.[/list_product_images]
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