Component of the anaphase promoting complex/cyclosome(APC/C), a cell cycle-regulated E3 ubiquitin ligase that controlsprogression through mitosis and the G1 phase of the cell cycle.The APC/C complex acts by mediating ubiquitination and subsequentdegradation of target proteins: it mainly mediates the formationof 'Lys-11'-linked polyubiquitin chains and, to a lower extent,the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitinchains..
[list_product_images]APC1 phospho S377 was detected in paraffin-embedded sections of pancreatic carcinoma cells at 60X using rabbit anti-APC1 phospho S377 Antigen Affinity purified polyclonal antibody (Catalog # P03471-1) at 5 μg/mL. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.|Western blot analysis of phosphorylated APC1 expression in lysate from asynchronous cells (lane 1) and lysate from cells treated with nocodazole (lane 2). Phosphorylated APC1 at 215KD was detected using rabbit anti-APC1 pS377 Antigen Affinity purified polyclonal antibody (Catalog # P03471-1) at 1:1000. While some phosphorylated APC1 is present in untreated cell, the amount of phosphorylated protein is increased in cell preparations arrested in mitosis.[/list_product_images]