CREB phospho peptide corresponding to amino acid residues 122-147 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
纯化方式
Anti-CREB pS133 was affinity purified from monospecific antiserum by immunoaffinity purification.
浓度
0.064 mg/mL by UV absorbance at 280 nm
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
CREB1, also known as cAMP responsive element binding protein 1, is a protein that in humans is encoded by the CREB1 gene. It is mapped to 2q33.3. This protein binds the cAMP response element, a DNA nucleotide sequence present in many viral and cellular promoters. The binding of CREB1 stimulates transcription. This protein is a CREB transcription factor that is a member of the leucine zipper family of DNA-binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway.
[list_product_images]CREB was detected in paraffin-embedded sections of tonsillar lymphocytes tissue using rabbit anti-CREB pS133 Antigen Affinity purified polyclonal antibody (Catalog # P00577) at 20 μg/mL. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.|Western blot analysis of phosphorylated CREB expression using rabbit anti-CREB pS133 Antigen Affinity purified polyclonal antibody (Catalog # P00577) at 1:500. Recombinant His-tagged human CREB was produced in E.coli and purified by metal affinity chromatography. An aliquot of purified CREB was phosphorylated in-vitro using Protein Kinase A and ATP. Western blot of control (-) and in-vitro phosphorylated CREB (+) was used to show that the antibody reacts specifically with the phosphorylated form. Personal Communication, R. Screaton, The Salk Institute for Biological Studies.|Western blot analysis of phosphorylated CREB expression using rabbit anti-CREB pS133 Antigen Affinity purified polyclonal antibody (Catalog # P00577) at 1:500. Recombinant His-tagged human CREB was produced in E.coli and purified by metal affinity chromatography. An aliquot of purified CREB was phosphorylated in-vitro using Protein Kinase A and ATP. western blot of control (-) and in-vitro phosphorylated CREB (+) was used to show that the antibody reacts specifically with the phosphorylated form. Personal Communication, Boss, J., Emory University School of Medicine, Atlanta, GA.[/list_product_images]