手机验证
0
0
0
0
单克隆
human
0
3-4周
博士德生物
WB,IHC
0
100μg
产品名称 | Plk1 phospho T210 Antibody |
---|---|
产品描述 | Anti-Plk1 phospho T210 Antibody validated for WB, IHC in Human. |
文献引用格式 | Plk1 phospho T210 Antibody (Boster Biological Technology, Wuhan, China. Catalog #P00182) |
应用范围 | WB, IHC |
抗体来源 | Rabbit |
抗体规格 | 100μg |
克隆 | Polyclonal |
---|---|
产品形态 | Liquid |
浓度 | 1.0 mg/mL by UV absorbance at 280 nm |
小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
基因名 | PLK1 |
---|---|
基因全名 | Serine/threonine-protein kinase PLK1 |
蛋白功能 | Serine/threonine-protein kinase that performs severalimportant functions throughout M phase of the cell cycle,including the regulation of centrosome maturation and spindleassembly, the removal of cohesins from chromosome arms, theinactivation of anaphase-promoting complex/cyclosome (APC/C)inhibitors, and the regulation of mitotic exit and cytokinesis.Polo-like kinase proteins acts by binding and phosphorylatingproteins are that already phosphorylated on a specific motifrecognized by the POLO box domains. Phosphorylates BORA,BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1,FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1,KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14,TOPORS, p73/TP73, TPT1 and WEE1. Plays a key role in centrosomefunctions and the assembly of bipolar spindles by phosphorylatingKIZ, NEDD1 and NINL. NEDD1 phosphorylation promotes subsequenttargeting of the gamma-tubulin ring complex (gTuRC) to thecentrosome, an important step for spindle formation.Phosphorylation of NINL component of the centrosome leads to NINLdissociation from other centrosomal proteins. Involved in mitosisexit and cytokinesis by phosphorylating CEP55, ECT2, KIF20A/MKLP2,CENPU, PRC1 and RACGAP1. Recruited at the central spindle byphosphorylating and docking PRC1 and KIF20A/MKLP2; creates its owndocking sites on PRC1 and KIF20A/MKLP2 by mediatingphosphorylation of sites subsequently recognized by the POLO boxdomains. Phosphorylates RACGAP1, thereby creating a docking sitefor the Rho GTP exchange factor ECT2 that is essential for thecleavage furrow formation. Promotes the central spindlerecruitment of ECT2. Plays a central role in G2/M transition ofmitotic cell cycle by phosphorylating CCNB1, CDC25C, FOXM1, CENPU,PKMYT1/MYT1, PPP1R12A/MYPT1 and WEE1. Part of a regulatory circuitthat promotes the activation of CDK1 by phosphorylating thepositive regulator CDC25C and inhibiting the negative regulatorsWEE1 and PKMYT1/MYT1. Also acts by mediating phosphorylation ofcyclin-B1 (CCNB1) on centrosomes in prophase. PhosphorylatesFOXM1, a key mitotic transcription regulator, leading to enhanceFOXM1 transcriptional activity. Involved in kinetochore functionsand sister chromatid cohesion by phosphorylating BUB1B/BUBR1,FBXO5/EMI1 and STAG2/SA2. PLK1 is high on non-attachedkinetochores suggesting a role of PLK1 in kinetochore attachmentor in spindle assembly checkpoint (SAC) regulation. Required forkinetochore localization of BUB1B. Regulates the dissociation ofcohesin from chromosomes by phosphorylating cohesin subunits suchas STAG2/SA2. Phosphorylates SGOL1: required for spindle polelocalization of isoform 3 of SGOL1 and plays a role in regulatingits centriole cohesion function. Mediates phosphorylation ofFBXO5/EMI1, a negative regulator of the APC/C complex duringprophase, leading to FBXO5/EMI1 ubiquitination and degradation bythe proteasome. Acts as a negative regulator of p53 familymembers: phosphorylates TOPORS, leading to inhibit the sumoylationof p53/TP53 and simultaneously enhance the ubiquitination andsubsequent degradation of p53/TP53. Phosphorylates thetransactivation domain of the transcription factor p73/TP73,leading to inhibit p73/TP73-mediated transcriptional activationand pro-apoptotic functions. Phosphorylates BORA, and therebypromotes the degradation of BORA. Contributes to the regulation ofAURKA function. Also required for recovery after DNA damagecheckpoint and entry into mitosis. Phosphorylates MISP, leading tostabilization of cortical and astral microtubule attachmentsrequired for proper spindle positioning (PubMed:8991084,PubMed:11202906, PubMed:12207013, PubMed:12447691,PubMed:12524548, PubMed:12738781, PubMed:12852856,PubMed:12939256, PubMed:14532005, PubMed:14734534,PubMed:15070733, PubMed:15148369, PubMed:15469984,PubMed:16198290, PubMed:16247472, PubMed:16980960,PubMed:17081991, PubMed:17351640, PubMed:17376779,PubMed:17617734, PubMed:18174154, PubMed:18331714,PubMed:18418051, PubMed:18477460, PubMed:18521620,PubMed:18615013, PubMed:19160488, PubMed:19351716,PubMed:19468300, PubMed:19468302, PubMed:19473992,PubMed:19509060, PubMed:19597481, PubMed:23455478,PubMed:23509069). Together with MEIKIN, acts as a regulator ofkinetochore function during meiosis I: required both for mono-orientation of kinetochores on sister chromosomes and protectionof centromeric cohesin from separase-mediated cleavage (Bysimilarity).. |
Tissue Specificity | Placenta and colon. |
Uniprot ID | PLK1: P53350 |
Serine/threonine-protein kinase PLK1, also known as polo-like kinase 1 (PLK-1) or serine/threonine-protein kinase 13(STPK13), is an enzyme that in humans is encoded by the PLK1 (polo-like kinase 1) gene. Plk1 is an early trigger for G2/M transition. It supports the functional maturation of the centrosome in late G2/early prophaseand establishment of the bipolar spindle. Also, Plk1 phosphorylates and activates cdc25C, a phosphatase that dephosphorylates and activates the cyclinB/cdc2 complex. Studies have shown that the loss of PLK1 expression can inducepro-apoptotic pathways and inhibit growth. Based on yeast and murine studies of meiosis, human PLK1 may also have a regulatory function in meiosis.
点击图片放大
[list_product_images]PLK1 was detected in paraffin-embedded sections of human breast carcinoma tumor tissue using rabbit anti-Plk1 pT210 Antigen Affinity purified polyclonal antibody (Catalog # P00182) at 1:200. Personal Communication, Alan Yen, LifeSpanBiosciences, Seattle, WA.|Western blot analysis of endogenous PLK1 protein expression in Mouse A20 whole cell lysate. PLK1 was detected using rabbit anti-Plk1 pT210 Antigen Affinity purified polyclonal antibody (Catalog # P00182) at 1:500. Comparison to a molecular weight marker indicates a band of ~68 kDa corresponding to Plk-1 protein. It is suggested to use a nuclear extract from synchronized cells to greatly increase the abundance of this protein in preparations. Personal communication Steven Pelech, Kinexus Inc. Vancouver, BC.|Western blot analysis of endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs. PLK1 was detected using rabbit anti-Plk1 pT210 Antigen Affinity purified polyclonal antibody (Catalog # P00182) at 1:1000 (panel A) and pan reactive anti-Plk-1 (panel B). Anti-Plk1 pT210 detects a single band corresponding to endogenous plk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in these mutants. Personal communication Hai Jiang, Northwestern Univ.|PLK1 was detected in paraffin-embedded sections of human colon carcinoma tumor tissue using rabbit anti-Plk1 pT210 Antigen Affinity purified polyclonal antibody (Catalog # P00182) at 1:200. Personal Communication, Alan Yen, LifeSpanBiosciences, Seattle, WA.[/list_product_images]
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。