Participates in at least several B-cell activationprocesses as well as of other cell types. It is a costimulator ofDNA-synthesis. It induces the expression of class II MHC moleculeson resting B-cells. It enhances both secretion and cell surfaceexpression of IgE and IgG1. It also regulates the expression ofthe low affinity Fc receptor for IgE (CD23) on both lymphocytesand monocytes..
Interleukin-4(IL-4), also knowns as a B-cell stimulatory factor1(BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5. The standard product used in this kit is recombinant human IL-4, consisting of 130 amino acids with the molecular mass of 14KDa.
[list_product_images]Western blot analysis of IL-4 expression in Human IL-4 50ng (lane 1). IL-4 at 17KD was detected using rabbit anti- IL-4 Biotin conjugated antibody (Catalog # B00230) at 1:1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).[/list_product_images]