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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Mouse
96T
货号 | EK0589 |
---|---|
产品名称 | Mouse VEGFR1 ELISA Kit |
产品描述 | For quantitative detection of mouse VEGFR1 in cell culture supernates and serum. |
免疫原 | NSO, Y23-E759 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | Flt1 |
基因全名 | FMS-like tyrosine kinase 1 |
Uniprot ID | P35969 |
指标别名 | FLT 1 | FLT1 | Flt-1 | sflt 1 | sFLT1 | Soluble VEGFR1 variant 2 | Soluble VEGFR1 variant 21 | sVEGFR1 | Tyrosine protein kinase FRT | Tyrosine-protein kinase FRT | VEGF Receptor 1 | VEGFR 1 | VEGFR-1 | VGFR1 |
背景资料 | Vascular endothelial growth factor receptor 1 is a protein that in humans is encoded by the FLT1 gene. Oncogene FLT belongs to the src gene family and is related to oncogene ROS. Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0589-CAP | 96-well plate precoated with anti- mouse VEGFR1 antibody | 1 |
EK0589-ST | Lyophilized recombinant mouse VEGFR1 standard | 10ng/tube×2 |
EK0589-DA | Biotinylated anti- mouse VEGFR1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 210 | 954 | 6138 | 191 | 959 | 6693 |
Standard deviation | 8.82 | 66.78 | 349.86 | 11.26 | 74.8 | 468.51 |
CV(%) | 4.2% | 7% | 5.7% | 5.9% | 7.8% | 7% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.016 | 0.070 | 0.122 | 0.234 | 0.450 | 0.909 | 1.536 | 2.249 |
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