Shuttle reporter plasmid to test expression of bleomycin resistance in transformants. [1] Expresses the mutant beta-tubulin gene conferring benomyl resistance. [1] A 2.5 kb SalI/HindIII fragment contains the complete tubulin gene and 5′ and 3′ flanking regions. [2] The order of the major features in this plasmid is:pUC8 – EcoRI – BamHI – am promoter – ble – XhoI – am 3′ region – tub-2 (benomyl resistance) – pUC8. [1] Restriction digests of the clone give the following sizes (kb): BamHI–5.1, 2.1; HindIII–4.0, 3.2; BamHI/HindIII–2.6, 2.5, 1.5, 0.65; BamHI/XhoI–3.0, 1.35, 1.2, 0.9, 0.8; SalI/HindIII–3.3, 2.6, 0.85, 0.55. (ATCC staff) A (GT)n repeat. (personal communication) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Aspergillus nidulans, Neurospora crassa, E.coli.