p-delta-blue（pdeltablue）

名称	编号	规格	价格	手册
p-delta-blue（pdeltablue）

质粒编号:
质粒名称: p-delta-blue
其他名称:
质粒大小（bp）: 3507
抗性: Ampicillin
质粒类型: Unspecified
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平:
蛋白标签:
测序引物:
GenBank号: M88655
其他属性: The insert to be cloned should have a phosphorylated 5′ blunt end with the first base corresponding to the first base of the first codon. (personal communication) Expression vector producing proteins with the one additional N-terminal methionine. A modified lacZ’ allows blue/white screening of colonies.[1] Constructed from pfXblue (ATCC 77333) by deletion of lambda cII and coagulation factor Xa protease recognition sequences (nt 824-943). [1] Inserts can be cloned into the vector using a filled-in NcoI site and a MCS restriction site matching the 3′ end of the insert. The vector supplies the AUG initiator codon. (personal communication) The order of the major features of the plasmid is: pMB1 ori – ampR – lambdaPL promoter – NcoI/MCS/HindIII/modified lacZ’. [1] Restriction digests of the plasmid give the following sizes (kb): BamHI–3.507; BglI–1.784, 1.723; PstI–2.072, 1.435; PvuI–2.063, 1.444; PvuII–2.852, 0.655; SacI–3.257, 0.25. (personal communication) Restriction digests of the clone give the following sizes (kb): BglI–1.75 (doublet); EcoRI–3.5; PstI–2.1, 1.45. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 208983 Hosts: E.coli, E.coli HB101. Related vectors: pfXblue.
质粒图谱:

质粒序列:

1	attatgtaag	cagacagttt	tattgttcat	gatgatatat	ttttatcttg	50
51	tgcaatgtaa	catcagagat	tttgagacac	aacgtggctt	tgttgaataa	100
101	atcgaacttt	tgctgagttg	aaggatcaga	tcacgcatct	tcccgacaac	150
151	gcagaccgtt	ccgtggcaaa	gcaaaagttc	aaaatcacca	actggtccac	200
201	ctacaacaaa	gctctcatca	accgtggctc	cctcacttct	ggctggatga	250
251	tggggcgatt	caggctggta	tgagtcagca	acaccttctt	cacgaggcag	300
301	acctcagcgc	cggtgatgcc	gacgatgcgt	ccggcgtaga	ggatctctca	350
351	cctaccaaac	aatgcccccc	tgcaaaaaat	aaattcatat	aaaaaacata	400
401	cagataacca	tctgcggtga	taaattatct	ctggcggtgt	tgacataaat	450
451	accactggcg	gtgatactga	gcacatcagg	caggacgcac	tgaccaccat	500
501	gaaggtgacg	ctcttaaaat	taagccctga	agaagggcag	cattcaaagc	550
551	agaaggcttt	ggggtgtgtg	atacgaaacg	aagcattggc	gagctcgccc	600
601	ctaaagatca	acaaggccga	aagatttttt	taactataaa	cgctgatgga	650
651	ggcgtttatg	cccaagaggt	aaagcccttc	ccgagtaaca	aaaaaacaac	700
701	agcataaata	accccgctct	tacacattcc	agccctgaaa	aagggcatca	750
751	aattaaacca	cacctatggt	gtatgcattt	atttgcatac	attcaatcaa	800
801	ttgttatcta	aggaaatact	tacccatggt	tacgaattcg	agctcggtac	850
851	ccggggatcc	tctagagtcg	acctgcaggc	atgcaagctt	ggcactggcc	900
901	gtcgttttac	aacgtcgtga	ctgggaaaac	cctggcgtta	cccaacttaa	950
951	tcgccttgca	gcacatcccc	ctttcgccag	ctggcgtaat	agcgaagagg	1000
1001	cccgcaccga	tcgcccttcc	caacagttgc	gcagcctgaa	tggcgaatgg	1050
1051	cgctttgcct	ggtttccggc	accagaagcg	gtgccggaaa	gctggctgga	1100
1101	gtgcgatctt	cctgaggccg	atacggtcgt	cgtcccctca	aactggcaga	1150
1151	tgcacggtta	cgatgcgccc	atctacacca	acgtaaccta	tcccattacg	1200
1201	gtcaatccgc	cgtttgttcc	cacggagaat	ccgacgggtt	gttactcgct	1250
1251	cacatttaat	gttgatgaaa	gctggctaca	ggaaggccag	acgcgaatta	1300
1301	tttttgatgg	cgttcctatt	ggttaaaaaa	tgagctgatt	taacaaaaat	1350
1351	ttaacgcgaa	ttttaacaaa	atattaacgt	ttacaattta	aatatttgct	1400
1401	tatacaatct	tcctgttttt	ggggcttttc	tgattatcaa	ccgcggtaca	1450
1451	tatgatggcg	taatcatggt	catagctgtt	tcctgtgtga	aattgttatc	1500
1501	cgctcacaat	tccacacaac	atacgagccg	gaagcataaa	gtgtaaagcc	1550
1551	tggggtgcct	aatgagtgag	ctaactcaca	ttaattgcgt	tgcgctcact	1600
1601	gcccgctttc	cagtcgggaa	acctgtcgtg	ccagctgcat	taatgaatcg	1650
1651	gccaacgcgc	ggggagaggc	ggtttgcgta	ttgggcgctc	ttccgcttcc	1700
1701	tcgctcactg	actcgctgcg	ctcggtcgtt	cggctgcggc	gagcggtatc	1750
1751	agctcactca	aaggcggtaa	tacggttatc	cacagaatca	ggggataacg	1800
1801	caggaaagaa	catgtgagca	aaaggccagc	aaaaggccag	gaaccgtaaa	1850
1851	aaggccgcgt	tgctggcgtt	tttccatagg	ctccgccccc	ctgacgagca	1900
1901	tcacaaaaat	cgacgctcaa	gtcagaggtg	gcgaaacccg	acaggactat	1950
1951	aaagatacca	ggcgtttccc	cctggaagct	ccctcgtgcg	ctctcctgtt	2000
2001	ccgaccctgc	cgcttaccgg	atacctgtcc	gcctttctcc	cttcgggaag	2050
2051	cgtggcgctt	tctcatagct	cacgctgtag	gtatctcagt	tcggtgtagg	2100
2101	tcgttcgctc	caagctgggc	tgtgtgcacg	aaccccccgt	tcagcccgac	2150
2151	cgctgcgcct	tatccggtaa	ctatcgtctt	gagtccaacc	cggtaagaca	2200
2201	cgacttatcg	ccactggcag	cagccactgg	taacaggatt	agcagagcga	2250
2251	ggtatgtagg	cggtgctaca	gagttcttga	agtggtggcc	taactacggc	2300
2301	tacactagaa	ggacagtatt	tggtatctgc	gctctgctga	agccagttac	2350
2351	cttcggaaaa	agagttggta	gctcttgatc	cggcaaacaa	accaccgctg	2400
2401	gtagcggtgg	tttttttgtt	tgcaagcagc	agattacgcg	cagaaaaaaa	2450
2451	ggatctcaag	aagatccttt	gatcttttct	acggggtctg	acgctcagtg	2500
2501	gaacgaaaac	tcacgttaag	ggattttggt	catgagatta	tcaaaaagga	2550
2551	tcttcaccta	gatcctttta	aattaaaaat	gaagttttaa	atcaatctaa	2600
2601	agtatatatg	agtaaacttg	gtctgacagt	taccaatgct	taatcagtga	2650
2651	ggcacctatc	tcagcgatct	gtctatttcg	ttcatccata	gttgcctgac	2700
2701	tccccgtcgt	gtagataact	acgatacggg	agggcttacc	atctggcccc	2750
2751	agtgctgcaa	tgataccgcg	agacccacgc	tcaccggctc	cagatttatc	2800
2801	agcaataaac	cagccagccg	gaagggccga	gcgcagaagt	ggtcctgcaa	2850
2851	ctttatccgc	ctccatccag	tctattaatt	gttgccggga	agctagagta	2900
2901	agtagttcgc	cagttaatag	tttgcgcaac	gttgttgcca	ttgctgcagg	2950
2951	catcgtggtg	tcacgctcgt	cgtttggtat	ggcttcattc	agctccggtt	3000
3001	cccaacgatc	aaggcgagtt	acatgatccc	ccatgttgtg	caaaaaagcg	3050
3051	gttagctcct	tcggtcctcc	gatcgttgtc	agaagtaagt	tggccgcagt	3100
3101	gttatcactc	atggttatgg	cagcactgca	taattctctt	actgtcatgc	3150
3151	catccgtaag	atgcttttct	gtgactggtg	agtactcaac	caagtcattc	3200
3201	tgagaatagt	gtatgcggcg	accgagttgc	tcttgcccgg	cgtcaacacg	3250
3251	ggataatacc	gcgccacata	gcagaacttt	aaaagtgctc	atcattggaa	3300
3301	aacgttcttc	ggggcgaaaa	ctctcaagga	tcttaccgct	gttgagatcc	3350
3351	agttcgatgt	aacccactcg	tgcacccaac	tgatcttcag	catcttttac	3400
3401	tttcaccagc	gtttctgggt	gagcaaaaac	aggaaggcaa	aatgccgcaa	3450
3451	aaaagggaat	aagggcgaca	cggaaatgtt	gaatactcat	actcttcctt	3500
3501	tttcaat	3507