The CellTiter-Glo® 3D Cell Viability Assay is designed for determining cell viability in 3D microtissue spheroids. The assay reagent penetrates large spheroids and has increased lytic capacity—allowing more accurate determination of viability compared to other assay methods. Based on the same reliable chemistry as the classic CellTiter-Glo® Assay, this new 3D assay reagent measures ATP as an indicator of viability and generates a luminescent readout that is much more sensitive than colorimetric or fluorescence-based methods. The simple, 30-minute protocol and ready-to-use reagent allows for fast results. A Cell Viability Assay Validated for 3D Microtissue Cultures
Accurate 3D cytotoxicity determination
Easy assay implementation
Simple, 30-minute protocol
Better Lytic Capacity Improved 3D Microtissue Penetration, More Accurate Viability Data HCT116 colon cancer spheroids were generated by seeding cells in the InSphero GravityPLUS™ 96-well hanging-drop platform and grown for 4 days. Panel A. An equivalent volume of reagent was added to all samples, and after 5 minutes of shaking, luminescence was recorded at 30 minutes. Panel B. A 2X concentration of CellTox™ Green Dye was added to CellTiter-Glo® 3D Reagent (left) or ATPlite™ 1Step Reagent (right) prior to sample addition as an indicator of cell lysis and images were acquired at 30 minutes. The spheroids in Panel B are ~300μm in diameter, and the bars in each image represent a distance of 200μm.