- ￥2800 - 3400
- Abcam
- 英国
- Ab22595
- 2025年07月12日
- WB, ICC/IF, IP
- 兔
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- 文献和实验
- 技术资料
- 免疫原：
Synthetic peptide corresponding to Human Calnexin aa 550 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
- 亚型：
IgG
- 形态：
液体
- 保存条件：
在 +4°C 下短期储存，在 -20°C 或 -80°C长期储存。 避免冻融循环
- 克隆性：
多克隆
- 库存：
大量
- 供应商：
北京百奥创新科技有限公司
- 宿主：
兔
- 应用范围：
WB, ICC/IF, IP
- 抗体英文名：
Anti-Calnexin antibody - ER Marker
- 抗体名：
Anti-Calnexin antibody - ER Marker
- 规格：
100ug
产品货号：Ab22595
产品规格：100ug
保存条件：在 +4°C 下短期储存，在 -20°C 或 -80°C长期储存。 避免冻融循环
形态：液体
应用范围：WB, ICC/IF, IP
保质期：按说明书条件可保存1-2年
Subcellular distribution of Smn and hnRNP R in isolated mouse embryonic motoneurons.
Lentiviral knockdown of hnRNP R led to a dose-dependent reduction of hnRNP R levels. Calnexin and Smn protein were not altered significantly.
Primary motoneurons or E18 spinal cord tissue, respectively, were lysed with cytosolic and nuclear fractionation buffer, solubilized in Laemmli buffer and boiled for 10 minutes at 99°C. Proteins were then subjected to SDS-PAGE, blotted onto PVDF membrane, incubated with the corresponding antibodies, including ab22595.
Calnexin was immunoprecipitated using 0.5 mg HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extract, 5 µg of Rabbit polyclonal to Calnexin - ER membrane marker and 50 µl of protein G magnetic beads (+).
No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10 minutes, HeLa whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10 minutes under agitation.
Proteins were eluted by addition of 40 µl SDS loading buffer and incubated for 10 minutes at 70oC; 10 µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab22595.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 80kDa: Calnexin - ER membrane marker.
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文献和实验Wang C et al. A light-activated magnetic bead strategy utilized in spatio-temporal controllable exosomes isolation. Front Bioeng Biotechnol 10:1006374 (2022).
4-Hydroxy-2-Nonenal (4-HNE) Staining by Anti-HNE Antibody
using an anti-4-HNE monoclonal antibody (MAb) (6 –8 ) and labeled goat anti-mouse IgG antibody (9 ).
祝贺〖雅裕睿安〗成为Signalway Antibody(SAB)独家
Biology International''31(6):638-644. 货号3011，产品描述：Goat anti-Rabbit IgG Secondary antibody unconjugated； 货号3012，产品描述：Goat anti-Rabbit IgG Secondary antibody HRP conjugated； 货号3021，产品描述：Goat anti-Human IgG
-phosphoserine的抗体。 摘抄： Anti-Phosphothreonine (MAb) - CryptateThe anti-phosphothreonine antibody is a monoclonal antibody (clone 42H4) produced at Cell Signaling Technology (reference 9386). It recognizes phosphorylated threonine residues in a manner
技术资料