- ￥5600
- GeneTex
- 美国
- GTX60996
- 2025年07月15日
- WB, ICC/IF, IHC-Fr
- Mouse
- Human, Mouse, Rat, Bovine, Pig, Horse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Full length recombinant human protein expressed in and purified from E. coli.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Bovine, Pig, Horse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX60996
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-Fr
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
c-Fos
- 抗体英文名：
c-Fos antibody [2H2]
- 抗体名：
c-Fos 抗体 [2H2]
- 规格：
100 μl
Left: GTX60996 staining (green) in HeLa cells, which were treated with serum-starvation for 36 hours, followed by 2 hours, 20% FBS stimulation (bottom panel), or PBS treatment (top panel). Green c-Fos staining only localizes in the nuclei of stimulated cells, but not in un-stimulated cells. Cells are counter-stained with our chicken polyclonal antibody against vimentin, CPCA-Vim, in red). Blue shows DAPI staining of nucleus．Middle: Mouse brain section (45 μM; fixed by transcardial perfusion with 4% PFA) labeled with GTX60996 using a standard HRP-DAB (horseradish peroxidase-3,3’-diaminobenzidine) staining technique. Cells expressing c-Fos show dark color in nucleus. Right: Mouse cortical section labeled with GTX60996 (red) and rabbit polyclonal anti Fox3/NeuN antibody (green) using immuno-fluorescent confocal-microscopy. Neurons positive for c-Fos and Fox3/NeuN appear to be yellow. Inset shows an enlarged image of GTX60996 staining. Nuclei are labeled with Dapi (blue).
Top panel: Western blot analysis of c-Fos expression in HeLa cells using GTX60996. Lane 1: HeLa cells were serum-starved for 36 hours. 2: Serum-starved HeLa cells were stimulated with 20% FBS (fetal bovine serum) for 2 hours. GTX60996 recognizes bands in the range of 50-65 kDa, which represent multiple forms of c-Fos. Serum starvation attenuates c-Fos expression, while 20% FBS strongly stimulates c-Fos expression. Bottom panel: Blot was stripped and probed with monoclonal antibody against GAPDH.
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文献和实验Chang CH et al., Cell Rep 2019 (PMID:31365864)
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人C-Fos ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 C-Fos 单抗包被于酶标板上，标准品和样品中的 C-Fos与单抗结合，加入生物素化的抗人 C-Fos ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠 C-Fos ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 C-Fos 单抗包被于酶标板上，标准品和样品中的 C-Fos 与单抗结合，加入生物素化的抗大鼠 C-Fos ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合，加入底物工作液显蓝
Antibody Staining of Ovaries 1. Dissect ovaries in EBR 2. Transfer ovaries into an Eppendorf tube and add 100 µl devitellinizing buffer and 600-µl heptane 3. Agitate gently for 10 minutes
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