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PI3-kinase p85- alpha (Phospho-Tyr607) Antibody
PI3-kinase p85- alpha
1.0mg/ml
WB
Rabbit
Hu Ms
Polyclonal
Store at -20℃
liquid
Peptide sequence around phosphorylation site of Tyrosine 607 (D-Q-Y(p)-S-L) derived from Human PI3-kinase p85-alpha.
50ul/50ul
规格: | 50ul | 产品价格: | 询价 |
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规格: | 50ul | 产品价格: | 询价 |
All Lanes: PI3-kinase p85- (Phospho-Tyr607) Antibody at 1/ 500 dilution.
Lane1: MCF7 serum starvation
Lane2: MCF7 treated with 20nM EGF for 15min
Lane3:MCF7 treated with 100nM Dasatinib for 30min
Lane4: MCF7 Serum Starvation, then treated with Lambda Protein Phosphatase
Lane5: MCF7 treated with 20nM EGF for 15min, then treated with Lambda Protein Phosphatase
Lane6:MCF7 treated with 100nM Dasatinib for 30min, then treated with Lambda Protein Phosphatase Lysates/ Proteins at 40ug per lane
Secondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilution
Predicted band size : 80kDa
Observed band size: 80kDa
All Lanes: PI3-kinase p85- (Phospho-Tyr607) Antibody at 1/ 500 dilution. Lane1: Rat kidney tissue lysate Lane2: Rat kidney tissue lysate, Primary antibody blocked with the 1ug/mL phospho peptide at 37°c for 2h Lysates/ Proteins at 60ug per lane Secondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilution,37°c 1h Predicted band size : 80kDa Observed band size: 80kDa
Immunohistochemical analysis of paraffin-embedded Human placenta. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1,PI 3-kinase p85α (phospho Tyr607) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,PI 3-kinase p85α (phospho Tyr607) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,PI 3-kinase p85α (phospho Tyr607) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemistry analysis of paraffin-embedded human brain, using PI3-kinase p85-alpha (Phospho-Tyr607) Antibody. The picture on the right is blocked with the phospho peptide.
All Lanes: PI3-kinase p85- (Phospho-Tyr607) Antibody at 1/ 500 dilution. Lane1: Mouse Brain tissue lysate Lane2: Mouse Liver tissue lysate Lane3: Mouse Spleen tissue lysate Lane4: Rat Lung tissue lysate Lane5: Rat Kidney tissue lysate Lane6: Rat Testis tissue lysate Lane7: Rat Ovary tissue lysate Lysates/ Proteins at 40ug per lane Secondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilution Predicted band size : 80kDa Observed band size: 80kDa
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