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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Peptide sequence around phosphorylation site of threonine360/serine 362 (V-P-T(p)-P-S(p)-P-L) derived from Human CK2a.
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Human
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB
- 靶点:
Uniprot:P68400
- 抗体英文名:
CK2a(phospho-Thr360/Ser362) Antibody
- 规格:
50ul/100ul
配方:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:
Predicted MW: 42kd
Western blotting: 1:1000
图片:

Western blot of CK2a(Phospho- Thr360/Ser362) antibody(#11572) and CK2a antibody(#21572) in vitro kinase assay. Both purified ERK2 and CK2 were used. CK2a(Phospho-Thr360/Ser362) antibody could recognize ERK2 phosphorylated wild type CK2a and CK2a when Ser362 was mutated to alanine .
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
4 ). 2. Other antibodies used were b-catenin (phospho-Thr41/Ser37/Ser33) (#9561; Cell Signaling Technology), b-catenin (#9562;Cell Signaling Technology), CRMP2 (pT514/509) (#PB-043; Kinasource, Dundee, Scotland) and CRMP2 (#AB-042;Kinasource). 3. Blocking buffer
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