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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Peptide sequence around phosphorylation site of serine 352 (R-D-S(p)-P-G) derived from Human p90RSK.
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Human;Mouse;Rat
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB
- 靶点:
Uniprot:Q15418
- 抗体英文名:
p90RSK(Phospho-Ser352) Antibody
- 规格:
50ul/100ul
配方:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:
Predicted MW: 90kd
Western blotting: 1:500~1:1000
图片:

Western blot analysis of extracts from HUVEC cells using p90RSK(Phospho-Ser352) Antibody #11113(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
,

Western blot analysis of extracts from Hela cells, treated with PMA or calf intestinal phosphatase (CIP), using p90RSK (Phospho-Ser352) Antibody #11113.
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文献和实验licanming 我的实验是用VEGF处理细胞后测定细胞PKC激活水平,拟采用Western blot的方法,用ECL法检测,这样分析PKC激活程度结果国际上认可吗? 我在Cell Signaling Technology上,查到 2261 Phospho-(Ser) PKC Substrate Antibody W IP E D 9371 Phospho-PKC (pan) (betaII Ser660) Antibody W IP
antibody dilution buffer: TBS-T supplemented with2% (w/v) BSA. 6. Antibodies: Phospho-b-catenin Ser33/Ser37/Thr41, phospho-glycogen synthase Ser641, and glycogen synthase (CellSignaling Technologies, Beverly, MA); b-catenin and GSK-3b(BD PharMingen, San
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
a pseudo-substrate and binds to the phosphate-binding pocketon GSK3, preventing interaction with primed substrates (8) . Phosphorylation at this site is mediated by members of the AGCfamily of kinases (e.g. PKB, PKC, p70S6K, and p90RSK) andcommonly
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