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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthesized Acetyl peptide derived from human Rb. at AA range: K873/872
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Human;Mouse;Rat
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB
- 靶点:
Uniprot:P01009
- 抗体英文名:
Rb (Acetyl-K873/K874) Polyclonal Antibody
- 规格:
50ul/100ul
计算分子量:106 kDa
配方:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
应用详情:Western blotting: 1£º500-1£º2000
图片:

Western Blot analysis of 1,mouse-heart 3,mouse-brain cells using primary antibody diluted at 1:1000(˚C overnight). Secondary antibody£oGoat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25??C, 1 hour)
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文献和实验in the extract with high affinity polyclonal antibodies, using anti-XKCM1 as an example.) Put 25 ul of bead slurry into two 0.5 ml tubes labeled IgG and XKCM1. Wash beads 3X with 0.5 ml TBST each wash. Add Rb IgG (4 ug) or anti-XKCM1 Gly
, Polyclonal and Monoclonal Antibody Production were provided by members of Gerry Rubin's Laboratory (Berkeley). Any comments in the methods (technical errors etc.) E-mail: d.bowtell@pmci.unimelb.edu.au David Bowtell PMCI October 1998
展研究 参考文献: Czerkinsky, C.C., L.A. Nilsson, H. Nygren, O. Ouchterlony, and A. Tarkowski. 1983. A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody-secreting cells. J. Immunol. Meth. 65: 109.
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