- 询价
- Signalway Antibody
- 35066
- 2025年09月22日
- WB
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthesized peptide derived from internal of human SLC4A8/10.
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB
- 靶点：
Uniprot：Q6U841
- 抗体英文名：
SLC4A8/10 Antibody
- 规格：
50ul/100ul
配方:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:Western blotting: 1:500~1:3000
图片:
Western blot analysis of extracts from COLO cells, using SLC4A8/10 antibody #35066.
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文献和实验8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
step 5 min Antibody 15 min PBS Wash 6 x 2 min Protein A Gold 15 min PBS Wash 6 x 2 min Water Wash 5 x 1 min. Blocking Any "sticky" protein can be used to cover non-specific binding sites on the thawed sections
, and incubate for 1 hour on ice. Pellet cells, giving an antibody supernatant. Add this to a cell pellet prepared as in step 9. Be very careful not to get supernatants mixed up! Repeat steps 9 + 10 seven times, or as often as necessary to decrease
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