- 询价
- Signalway Antibody
- 44006
- 2025年09月22日
- WB IP IF
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic peptide:HHHHHH conjugated to KLH.
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Monoclonal
- 保质期：
12 months
- 抗原来源：
Mouse
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Mouse
- 应用范围：
WB IP IF
- 抗体英文名：
His-Tag Monoclonal Antibody(4E6)
- 规格：
50ul/100ul
应用详情:
Western blotting: 1:3000
Immunoprecipitation: 1:200
Immunofluorescence: 1:1000
图片:
IF analysis of 293 cells transfected with a His-tag protein,1:1000 dilution (blue DAPI, red anti-His)
,
2ug His fusion protein+ Primary antibody dilution at 1) 1:5000 2) 1:10000
,
IP antibody use: 5ug His Mouse IgG1 per ml Lysate, WB 1: 3000 1) untransfected 293 cell lysate 2) transfected 293 cell lysate with His-tag fusion protein 3) IP (untransfected 293+anti-His mAb+ Protein G agarose) 4) IP (transfected 293+ normal Mouse IgG
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文献和实验Polyclonal and Monoclonal Antibodies
later again (2 + 2 week cycle). Monoclonal Antibody If titre is positive do one of the following 2 weeks later: I - Boost tail vein with 20 ug- 50 ug of Antigen in PBS and proceed with fusion on day 4. OR II - Boost
. Goding. 3. Monoclonal antibody technology; Ailsa M. Campbell. 上一篇：monoclonal antibody production fusion 下一篇：Polyclonal and Monoclonal Antibodies
Expanding Hybridoma（杂交瘤细胞） Clones
that the subclone is monoclonal, checking the isotype is possible . If not convinced that the hybrydoma is monoclonal, reclone a second time. Cloning of Hybridoma Make an accurate cell count of cells in log phase (3 E5 - 1E6 cells / ml).
技术资料暂无技术资料 索取技术资料
