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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Taraxacum officinale 2S albumin, partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥15792.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥4464.0 |
| 规格: | 20μg | 产品价格: | ¥2832.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
/Uniprot No.:
P86783别名:
To-A1种属:
Taraxacum officinale (Common dandelion) (Leontodon taraxacum)蛋白长度:
Partial来源:
E.coli分子量:
2.3 kDa表达区域:
1-19aa氨基酸序列:
PVSRQQCSQRIQGERFNQC蛋白标签:
Tag-Free产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
This is a 2S seed storage protein. Has antifungal activity. Inhibits spore germination in H.sativum (IC50=62.5 µg/ml) and P.betae (IC50=62.5 µg/ml). Inhibits growth of H.sativum, V.albo-atrum and P.infestans.内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
OthersReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
"Antifungal activity of storage 2S albumins from seeds of the invasive weed dandelion Taraxacum officinale Wigg." Odintsova T.I., Rogozhin E.A., Sklyar I.V., Musolyamov A.K., Kudryavtsev A.M., Pukhalsky V.A., Smirnov A.N., Grishin E.V., Egorov T.A. Protein Pept. Lett. 17:522-529(2010)Function:
This is a 2S seed storage protein. Has antifungal activity. Inhibits spore germination in H.sativum (IC(50)=62.5 ug/ml) and P.betae (IC(50)=62.5 ug/ml). Inhibits growth of H.sativum, V.albo-atrum and P.infestans.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验+XxVaYaITMsF3SAN8uDmpP6kjSEisDch8Nu/Ce1NUkZZIgIRgYhARCAisN8igBkCOly5YUtVrKRZeyl3iyx7BxHHCssxS+E8RET2mp/8yP7uK1+x08460/7tuuvskne/U2S6am1yFffCc09r9by0fCSX7Z67FuGBzXq7u62g5a2LmqzXFGmFGFdlkpHS/pAhQ6xXPppz8o+MBKVekWm5iSvLTRzCwFnxPoHVxRFHHOGE+oMf/pCde+45NnHieFu48GY
之前首先被洗脱,糖化Hb可分为HbA1a(其中与1,6-二磷酸果糖结合的为HbA1a1;与6-磷酸葡萄糖结合的为HbA1a2),HbA1b(尚无明确定义,包括HbA1b1,HbA1b2,HbA1b3),HbA1e(与葡萄糖结合)和HbA1d(α链和β链内氨基以及α链的N末端基团上的烯化血红蛋白;包括HbA1d1,HbA1d2,HbA1d3)共九种。最重要的是HbA1c.HbA1e的生成量取决于血糖的浓度,正常人约占4%~6%。由于红细胞的半寿期是60天,所以GHb的测定可以反映测定前8周左右病人
【共享】 蛋白质含量测定法/蛋白质沉淀法/Western免疫印迹
。(2)仍有一些物质干扰此法的测定,主要的干扰物质有:去污剂、 Triton X-100、十二烷基硫酸钠(SDS)和0.1N的NaOH。(如同0.1N的酸干扰Lowary法一样)。(3)标准曲线也有轻微的非线性,因而不能用Beer定律进行计算,而只能用标准曲线来测定未知蛋白质的浓度。(二)试剂与器材1. 试剂:(1)标准蛋白质溶液,用 g—球蛋白或牛血清清蛋白(BSA),配制成1.0mg/ml和0.1mg/ml的标准蛋白质溶液。(2)考马斯亮兰G—250染料试剂:称100mg考马斯亮兰G—250,溶于50
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