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- CLOUD-CLONE CORP, KLSW
- 美国
- klRPF074Mu01
- 2025年07月16日
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- 详细信息
- 技术资料
- 保存条件：
负20度
- 英文名：
Recombinant Profilin 3 (PFN3)
- 库存：
大量
- 供应商：
柯雷生物
[ PROPERTIES ]
Residues: Met1~Leu132
Tags: Two N-terminal Tags, His-tag and GST-tag
Accession: Q9DAD6
Host: E. coli
Subcellular Location: Cytoplasm, cytoskeleton. Nucleus.
Purity: >90% Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 8.9
Predicted Molecular Mass: 44.1kDa
Applications: SDS-PAGE; WB; ELISA; IP. (May be suitable for use in other assays to be determined by the end user.) [ USAGE ] Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months. Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
MSDWKGYISA VLRDQRIDDV AIVGHSDNRC VWASRPGGLL AAISPQEVGV LTGPDRHTFL QTGLSVAGRR CCVIRDYLLA EGDGVLDART KGLDGRAICV GHTPRALLVL MGRRGVHGGI LNKTVHDLIG GL
[ REFERENCES ]
1. Braun A., et al. (2002) Gene 283:219-225.
2. Hara Y., et al. (2008) FEBS Lett. 582:2998-3004.
3. Carlsson L., et al. (1977) J. Mol. Biol. 115 (3): 465–83
Residues: Met1~Leu132
Tags: Two N-terminal Tags, His-tag and GST-tag
Accession: Q9DAD6
Host: E. coli
Subcellular Location: Cytoplasm, cytoskeleton. Nucleus.
Purity: >90% Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 8.9
Predicted Molecular Mass: 44.1kDa
Applications: SDS-PAGE; WB; ELISA; IP. (May be suitable for use in other assays to be determined by the end user.) [ USAGE ] Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months. Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
MSDWKGYISA VLRDQRIDDV AIVGHSDNRC VWASRPGGLL AAISPQEVGV LTGPDRHTFL QTGLSVAGRR CCVIRDYLLA EGDGVLDART KGLDGRAICV GHTPRALLVL MGRRGVHGGI LNKTVHDLIG GL
[ REFERENCES ]
1. Braun A., et al. (2002) Gene 283:219-225.
2. Hara Y., et al. (2008) FEBS Lett. 582:2998-3004.
3. Carlsson L., et al. (1977) J. Mol. Biol. 115 (3): 465–83
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抑丝蛋白3(PFN3)重组蛋白
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