ChRM2/Acetylcholine receptor(M

化学遗传学技术原理及其应用

DREADDs受体作用原理（Scott M. Sternson & Bryan L. Roth, Annu. Rev. Neurosci., 2014）2. 常见的DREADDs受体（1）Gq-DREADD和hM3Dq：最初的Gq-DREADD即hM3Dq，改造自人毒蕈碱型乙酰胆碱受体（the human muscarinic acetylcholine receptor，mAchRs）亚型M3（也称为hM3）。在正常生理状况下，hM3和乙酰胆碱结合，然后和Gq类G蛋白耦合受体耦合，作用于磷脂酶C、肌醇三磷酸

Overview of Receptor Allosterism

Figure 1.21.1 Allosterism between a receptor and its G protein: the effects of GTP on the competition between [3 H] N ‐methylscopolamine and the agonist, carbachol, at the muscarinic acetylcholine M2 receptor in rat myocardial membranes. Competition

Opioid Receptor Coupling to GIRK Channels: In Vitro Studies Using a Xenopus Oocyte Expression System and In Vivo Studies on Weaver Mutant Mice

have been shown to be involved in opioid-induced analgesia (2 ). These channels are activated by G protein-coupled receptors (GPCRs) such as opioid, nociceptin/orphanin FQ, M2 muscarinic, α2 adrenergic, and D2 dopaminergic receptors via the βγ subunits of G