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文献和实验Pulmonary Artery Endothelial Cells
the culture of endothelial cells isolated from human umbilical veins. Ryan and coworkers (8 ) described, in 1978, the first isolation of pulmonary artery endothelial cells. Porcine endothelial cells have been used to study endothelial permeability in vitro
Isolation and Culture of Primary Endothelial Cells
The purpose of this chapter is to describe the isolation techniques that result in pure cultures of human vascular endothelial cells from the umbilical vein and umbilical cord blood. We first describe the isolation of human umbilical vein
Transfection and Transduction of Primary Human Endothelial Cells
The endothelium is involved in a number of normal physiological processes (regulating circulating levels of vasoactive agents, blood/gas exchange, regulating cellular traffic between intavascular and extravascular compartments of tissues
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