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文献和实验Culture of Primary Human Bronchial Epithelial Cells
The human airway is normally kept sterile despite continual exposure to airborne pathogens. This is achieved by host defense mechanisms that exist to prevent adherence, colonization, and invasion of the airway epithelium. The airway
Isolation and Culture of Human Alveolar Epithelial Cells
will concentrate on the isolation and culture of human alveolar epithelial cells that line the peripheral gas exchange region of the lung. Whereas immortalized cell lines emanating mostly from the different cells of the tracheal/bronchial epithelium of human
Primary Culture and Propagation of Human Prostate Epithelial Cells
can be technically challenging, even for laboratories experienced in immortalized cell culture. Therefore, we provide methods to isolate and culture primary epithelial cells directly from human prostate tissue. Initially, we describe the isolation of bulk epithelial
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