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文献和实验Isolation and Culture of Primary Endothelial Cells
The purpose of this chapter is to describe the isolation techniques that result in pure cultures of human vascular endothelial cells from the umbilical vein and umbilical cord blood. We first describe the isolation of human umbilical vein
Transfection and Transduction of Primary Human Endothelial Cells
The endothelium is involved in a number of normal physiological processes (regulating circulating levels of vasoactive agents, blood/gas exchange, regulating cellular traffic between intavascular and extravascular compartments of tissues
Transfection of Indoleamine 2,3 Dioxygenase in Primary Endothelial Cells
to the endothelium. We show that IDO can be readily transfected in primary endothelial cells and detail a method to determine the activity of the transfected protein.
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