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文献和实验In Vitro Models of Early Neoplastic Transformation of Human Mammary Epithelial Cells
About 80% of all human cancers are carcinomas, representing oncogenic transformation of epithelial cells. In-vitro models of epithelial cell transformation are therefore of great interest to elucidate the molecular mechanisms of human cancer
Culture of Primary Human Bronchial Epithelial Cells
The human airway is normally kept sterile despite continual exposure to airborne pathogens. This is achieved by host defense mechanisms that exist to prevent adherence, colonization, and invasion of the airway epithelium. The airway
Three-Dimensional Cultures of Mouse Mammary Epithelial Cells
and architecture. The accompanying chapter of Vidi et al. describes 3D models developed for human cells. Here, we describe how mouse primary epithelial cells and cell lines—essentially those we use in our laboratory—are cultured in relevant 3D microenvironments
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