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- cusab
- CSB-PA00025A0Rb
- 2025年07月14日
- ELISA,WB,IHC,IF;Recommended dilution:WB:1:2000-1:5000,IHC:1:20-1:200,IF:1:50-1:200
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 克隆性：
多克隆
- 供应商：
cusab
- 应用范围：
ELISA,WB,IHC,IF;Recommended dilution:WB:1:2000-1:5000,IHC:1:20-1:200,IF:1:50-1:200
- 浓度：
100ug
- 靶点：
GAPDH
- 抗体英文名：
GAPDH Antibody
- 抗体名：
GAPDH Antibody
- 规格：
100ug
- 产品类型:
Polyclonal Antibody
- 产品名称:
GAPDH Antibody
- 英文名称:
GAPDH Antibody
- 货号:
CSB-PA00025A0Rb
- 规格:
- 别名:
GAPDH, GAPD, CDABP0047, OK/SW-cl.12,Peptidyl-cysteine S-nitrosylase GAPDH
- 种属:
Human
- 免疫原:
Recombinant human Glyceraldehyde-3-phosphate dehydrogenase protein (3-105AA)
- 宿主:
Rabbit
- 种属反应性:
Human,mouse,Zebrafish
- 应用范围:
ELISA,WB,IHC,IF;Recommended dilution:WB:1:2000-1:5000,IHC:1:20-1:200,IF:1:50-1:200
- 背景:
Glycerol 3 Phosphate Dehydrogenase is a homodimer and belongs to the NAD dependent Glycerol 3 Phosphate Dehydrogenase family.
- 克隆类型:
Polyclonal
- 抗体亚型:
IgG
- 纯化方式:
Caprylic Acid Ammonium Sulfate Precipitation purified
- 标记方式:
Non-conjugated
- 保存缓冲液:
Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
- 产品提供形式:
Liquid
- 储存条件:
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
- 验证图:
Western blot
IHC
Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate
All lanes: GAPDH antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 37, 32 kDa
Immunohistochemistry of paraffin-embedded mouse liver using CSB-PA00025A0Rb at dilution 1:50
Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA00025A0Rb at dilution of 1:100
Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA00025A0Rb at dilution of 1:100
IF
Immunofluorescent analysis of MCF-7 cells using CSB-PA00025A0Rb at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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- 作者
- 内容
- 询问日期
文献和实验Glucagon Enhances Chemotherapy Efficacy By Inhibition of Tumor Vessels in Colorectal Cancer Y Xu,Advanced science,2023
Clinical, prognostic, and therapeutic significance of heat shock protein 27 in bladder cancer Lee MS,Oncotarget,2023
Downregulation of DNA methylation enhances differentiation of THP-1 cells and induces M1 polarization of differentiated macrophages J Park,Scientific reports,2023
Fatty acid oxidation supports melanoma cell migration through autophagy regulation S Shin,Biochemical and biophysical research communications,2023
Neuronal growth regulator 1 promotes adipocyte lipid trafficking via interaction with CD36 A Yoo,Journal of lipid research,2022
Mitochondrial glutamine metabolism regulates sensitivity of cancer cells after chemotherapy via amphiregulin S Hwang,Cell death discovery,2021
AC-motif: a DNA motif containing adenine and cytosine repeat plays a role in gene regulation JH Hur,Nucleic acids research,2021
Heterogeneous nuclear ribonucleoprotein A2/B1 regulates the ERK and p53/HDM2 signaling pathways to promote the survival, proliferation and migration of non‑small cell lung cancer cells Min Kyu Kim,Oncology Reports,2021
Mechanotransduction activates RhoA in the neiors of apoptotic epithelial cells to engage extrusion Kinga Duszyc,Current Biology,2021
Mesenchymal epithelial transition factor regulates tumor necrosis factor-related apoptotic induction ligand resistance in hepatocellular carcinoma cells through down-regulation of cyclin B1 S Lv,The International Journal of Biochemistry & Cell Biology,2020
Association Analysis of Single-Cell RNA Sequencing and Proteomics Reveals a Vital Role of Ca2+ Signaling in the Determination of Skeletal Muscle Development Potential. K Qiu,Cells,2020
Neuronal growth regulator 1-deficient mice show increased adiposity and decreased muscle mass Yeonhee Joo,International Journal of Obesity,2019
Phosphoregulation of the oncogenic protein regulator of cytokinesis 1 (PRC1) by the atypical CDK16/CCNY complex Hernandez-Ortega S, et al,Experimental & Molecular Medicine,2019
Evaluation of the Inhibitory Effects of Genipin on the Fluoxetine-Induced Invasive and Metastatic Model in Human HepG2 Cells Tian YS, et al,Molecules,2018
Ganoderma tsugae induced ROS-independent apoptosis and cytoprotective autophagy in human chronic myeloid leukemia cells Hseu YC, et al,Food and chemical toxicology,2018
Galectin-1 promotes the invasion of bladder cancer urothelia through their matrix milieu Ajay Balakrishnan.et al,biorxiv,2018
Progesterone Receptor Membrane Component 1 suppresses the p53 and Wnt/β-catenin pathways to promote human pluripotent stem cell self-renewal Kim JY.et al,Sci Rep,2018
GAPDH抗体|GAPDH抗体大全-艾美捷指导您购买合适的GAPDH抗体
，ProteinTech的GAPDH Monoclonal Antibody（货号60004-1-Ig）则是另一款单克隆抗体，除了WB和IF外，还可以应用于免疫组化IHC等实验。需要注意的是IF也是GAPDH抗体常用的应用类型之一。左图为ProteinTech的GAPDH Monoclonal Antibody（货号60004-1-Ig）抗体在石蜡包埋、福尔马林固定的切片上的应用。 更多GAPDH内参抗体 以及其他如Actin、Tubulin等其他内参抗体，请电话垂询。美国EarthOx品牌是一 家提供高性
【共享】进口内参抗体——在WB实验中为什么需要使用内参抗体？
进口内参抗体——在WB实验中为什么需要使用内参抗体？ 要检测一个基因的表达产物是否正确，或者比较表达产物量的相对变化，首选方法是Western Blot。虽然，顺利的时候Western Blot做起来很简单，可不顺的时候也很令人心烦――做不出结果啦、假阳性啦、结果出现多条带啦、到底是一抗有问题还是二抗有问题啦……毕竟，作为一种有活性的生物大分子，抗体和抗原的反应毕竟不象1+1那么明确，而用这种不确定的试剂来测定同样知之甚少的表达产物，确实是有一定的不确定性的。所以，严谨的Western
在WB实验中为什么需要使用内参抗体？ 进口内参抗体――在WB实验中为什么需要使用内参抗体？ 要检测一个基因的表达产物是否正确，或者比较表达产物量的相对变化，首选方法是Western Blot。虽然，顺利的时候Western Blot做起来很简单，可不顺的时候也很令人心烦做不出结果啦、假阳性啦、结果出现多条带啦、到底是一抗有问题还是二抗有问题啦……毕竟，作为一种有活性的生物大分子，抗体和抗原的反应毕竟不象1+1那么明确，而用这种不确定的试剂来测定同样知之甚少的表达产物，确实
技术资料
