PX377载体

PX377载体

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  • ZYbscience
  • 中国/美国
  • ZY68314
  • 2025年06月30日
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    上海泽叶生物科技有限公司
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    • 详细信息
    • 技术资料
    • 保存条件：

      -20℃低温保存

    • 保质期：

      三年

    • 英文名：

      PX377

    • 库存：

      20

    • 供应商：

      泽叶生物

    载体基本信息

    出品公司: ZYbscience
    载体名称: PX377 或 PX377 Corynebacter diphtheriae Cas9
    质粒类型: CRISPR-Cas9载体；基因敲除载体；昆虫细胞载体
    高拷贝/低拷贝: 高拷贝
    插入位点: 限制性内切酶，多克隆位点
    启动子: CMV
    载体大小: 10448bp
    5' 测序引物及序列: CMV Forward:CGCAAATGGGCGGTAGGCGTG
    3' 测序引物及序列: cctagtcagacaaaatgatgcaa
    载体标签: NLS (C ter)；HA、NLS (N ter)
    载体抗性: 氨苄青霉素
    筛选标记: Neomycin
    克隆菌株: Stbl3
    宿主细胞（系）: 昆虫细胞
    备注: Corynebacter diphtheriae Cas9
    产品目录号: ZY68314
    稳定性: 稳表达
    组成型/诱导型: 组成型
    病毒/非病毒: 杆状病毒

    载体质粒图谱和多克隆位点信息

    PX377载体图谱


    载体简介

    载体序列

    LOCUS       PX377 Corynebacter diphtheriae Cas9	10448 bp 	DNA	SYN
DEFINITION  PX377 Corynebacter diphtheriae Cas9
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..10448
                     /organism="PX377 Corynebacter diphtheriae Cas9"
                     /mol_type="other DNA"
     misc_feature    47..1001
                     /label="Ac_5_flank"
     CDS             complement(343..948)
                     /label="ORF frame 3"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1023..1587
                     /label="CMV_immearly_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1090..1377
                     /label="CAG_enhancer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1544..1564
                     /label="CMV_fwd_primer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1615..1633
                     /label="T7_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1633..1656
                     /label="lacO"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1772..1796
                     /label="attB1"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     CDS             1822..5139
                     /label="ORF frame 1"
                     /translation="MYPYDVPDYASPKKKRKVEASMKYHVGIDVGTFSVGLAAIEVDD
                     AGMPIKTLSLVSHIHDSGLDPDEIKSAVTRLASSGIARRTRRLYRRKRRRLQQLDKFI
                     QRQGWPVIELEDYSDPLYPWKVRAELAASYIADEKERGEKLSVALRHIARHRGWRNPY
                     AKVSSLYLPDGPSDAFKAIREEIKRASGQPVPETATVGQMVTLCELGTLKLRGEGGVL
                     SARLQQSDYAREIQEICRMQEIGQELYRKIIDVVFAAESPKGSASSRVGKDPLQPGKN
                     RALKASDAFQRYRIAALIGNLRVRVDGEKRILSVEEKNLVFDHLVNLTPKKEPEWVTI
                     AEILGIDRGQLIGTATMTDDGERAGARPPTHDTNRSIVNSRIAPLVDWWKTASALEQH
                     AMVKALSNAEVDDFDSPEGAKVQAFFADLDDDVHAKLDSLHLPVGRAAYSEDTLVRLT
                     RRMLSDGVDLYTARLQEFGIEPSWTPPTPRIGEPVGNPAVDRVLKTVSRWLESATKTW
                     GAPERVIIEHVREGFVTEKRAREMDGDMRRRAARNAKLFQEMQEKLNVQGKPSRADLW
                     RYQSVQRQNCQCAYCGSPITFSNSEMDHIVPRAGQGSTNTRENLVAVCHRCNQSKGNT
                     PFAIWAKNTSIEGVSVKEAVERTRHWVTDTGMRSTDFKKFTKAVVERFQRATMDEEID
                     ARSMESVAWMANELRSRVAQHFASHGTTVRVYRGSLTAEARRASGISGKLKFFDGVGK
                     SRLDRRHHAIDAAVIAFTSDYVAETLAVRSNLKQSQAHRQEAPQWREFTGKDAEHRAA
                     WRVWCQKMEKLSALLTEDLRDDRVVVMSNVRLRLGNGSAHKETIGKLSKVKLSSQLSV
                     SDIDKASSEALWCALTREPGFDPKEGLPANPERHIRVNGTHVYAGDNIGLFPVSAGSI
                     ALRGGYAELGSSFHHARVYKITSGKKPAFAMLRVYTIDLLPYRNQDLFSVELKPQTMS
                     MRQAEKKLRDALATGNAEYLGWLVVDDELVVDTSKIATDQVKAVEAELGTIRRWRVDG
                     FFSPSKLRLRPLQMSKEGIKKESAPELSKIIDRPGWLPAVNKLFSDGNVTVVRRDSLG
                     RVRLESTAHLPVTWKVQ*"
     misc_feature    1825..1851
                     /label="HA"
                     /translation="MYPYDVPDYASPKKKRKVEASMKYHVGIDVGTFSVGLAAIEVDD
                     AGMPIKTLSLVSHIHDSGLDPDEIKSAVTRLASSGIARRTRRLYRRKRRRLQQLDKFI
                     QRQGWPVIELEDYSDPLYPWKVRAELAASYIADEKERGEKLSVALRHIARHRGWRNPY
                     AKVSSLYLPDGPSDAFKAIREEIKRASGQPVPETATVGQMVTLCELGTLKLRGEGGVL
                     SARLQQSDYAREIQEICRMQEIGQELYRKIIDVVFAAESPKGSASSRVGKDPLQPGKN
                     RALKASDAFQRYRIAALIGNLRVRVDGEKRILSVEEKNLVFDHLVNLTPKKEPEWVTI
                     AEILGIDRGQLIGTATMTDDGERAGARPPTHDTNRSIVNSRIAPLVDWWKTASALEQH
                     AMVKALSNAEVDDFDSPEGAKVQAFFADLDDDVHAKLDSLHLPVGRAAYSEDTLVRLT
                     RRMLSDGVDLYTARLQEFGIEPSWTPPTPRIGEPVGNPAVDRVLKTVSRWLESATKTW
                     GAPERVIIEHVREGFVTEKRAREMDGDMRRRAARNAKLFQEMQEKLNVQGKPSRADLW
                     RYQSVQRQNCQCAYCGSPITFSNSEMDHIVPRAGQGSTNTRENLVAVCHRCNQSKGNT
                     PFAIWAKNTSIEGVSVKEAVERTRHWVTDTGMRSTDFKKFTKAVVERFQRATMDEEID
                     ARSMESVAWMANELRSRVAQHFASHGTTVRVYRGSLTAEARRASGISGKLKFFDGVGK
                     SRLDRRHHAIDAAVIAFTSDYVAETLAVRSNLKQSQAHRQEAPQWREFTGKDAEHRAA
                     WRVWCQKMEKLSALLTEDLRDDRVVVMSNVRLRLGNGSAHKETIGKLSKVKLSSQLSV
                     SDIDKASSEALWCALTREPGFDPKEGLPANPERHIRVNGTHVYAGDNIGLFPVSAGSI
                     ALRGGYAELGSSFHHARVYKITSGKKPAFAMLRVYTIDLLPYRNQDLFSVELKPQTMS
                     MRQAEKKLRDALATGNAEYLGWLVVDDELVVDTSKIATDQVKAVEAELGTIRRWRVDG
                     FFSPSKLRLRPLQMSKEGIKKESAPELSKIIDRPGWLPAVNKLFSDGNVTVVRRDSLG
                     RVRLESTAHLPVTWKVQ*"
     misc_feature    1855..1875
                     /label="SV40 NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMKYHVGIDVGTFSVGLAAIEVDD
                     AGMPIKTLSLVSHIHDSGLDPDEIKSAVTRLASSGIARRTRRLYRRKRRRLQQLDKFI
                     QRQGWPVIELEDYSDPLYPWKVRAELAASYIADEKERGEKLSVALRHIARHRGWRNPY
                     AKVSSLYLPDGPSDAFKAIREEIKRASGQPVPETATVGQMVTLCELGTLKLRGEGGVL
                     SARLQQSDYAREIQEICRMQEIGQELYRKIIDVVFAAESPKGSASSRVGKDPLQPGKN
                     RALKASDAFQRYRIAALIGNLRVRVDGEKRILSVEEKNLVFDHLVNLTPKKEPEWVTI
                     AEILGIDRGQLIGTATMTDDGERAGARPPTHDTNRSIVNSRIAPLVDWWKTASALEQH
                     AMVKALSNAEVDDFDSPEGAKVQAFFADLDDDVHAKLDSLHLPVGRAAYSEDTLVRLT
                     RRMLSDGVDLYTARLQEFGIEPSWTPPTPRIGEPVGNPAVDRVLKTVSRWLESATKTW
                     GAPERVIIEHVREGFVTEKRAREMDGDMRRRAARNAKLFQEMQEKLNVQGKPSRADLW
                     RYQSVQRQNCQCAYCGSPITFSNSEMDHIVPRAGQGSTNTRENLVAVCHRCNQSKGNT
                     PFAIWAKNTSIEGVSVKEAVERTRHWVTDTGMRSTDFKKFTKAVVERFQRATMDEEID
                     ARSMESVAWMANELRSRVAQHFASHGTTVRVYRGSLTAEARRASGISGKLKFFDGVGK
                     SRLDRRHHAIDAAVIAFTSDYVAETLAVRSNLKQSQAHRQEAPQWREFTGKDAEHRAA
                     WRVWCQKMEKLSALLTEDLRDDRVVVMSNVRLRLGNGSAHKETIGKLSKVKLSSQLSV
                     SDIDKASSEALWCALTREPGFDPKEGLPANPERHIRVNGTHVYAGDNIGLFPVSAGSI
                     ALRGGYAELGSSFHHARVYKITSGKKPAFAMLRVYTIDLLPYRNQDLFSVELKPQTMS
                     MRQAEKKLRDALATGNAEYLGWLVVDDELVVDTSKIATDQVKAVEAELGTIRRWRVDG
                     FFSPSKLRLRPLQMSKEGIKKESAPELSKIIDRPGWLPAVNKLFSDGNVTVVRRDSLG
                     RVRLESTAHLPVTWKVQ*"
     misc_feature    1861..1875
                     /label="NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMKYHVGIDVGTFSVGLAAIEVDD
                     AGMPIKTLSLVSHIHDSGLDPDEIKSAVTRLASSGIARRTRRLYRRKRRRLQQLDKFI
                     QRQGWPVIELEDYSDPLYPWKVRAELAASYIADEKERGEKLSVALRHIARHRGWRNPY
                     AKVSSLYLPDGPSDAFKAIREEIKRASGQPVPETATVGQMVTLCELGTLKLRGEGGVL
                     SARLQQSDYAREIQEICRMQEIGQELYRKIIDVVFAAESPKGSASSRVGKDPLQPGKN
                     RALKASDAFQRYRIAALIGNLRVRVDGEKRILSVEEKNLVFDHLVNLTPKKEPEWVTI
                     AEILGIDRGQLIGTATMTDDGERAGARPPTHDTNRSIVNSRIAPLVDWWKTASALEQH
                     AMVKALSNAEVDDFDSPEGAKVQAFFADLDDDVHAKLDSLHLPVGRAAYSEDTLVRLT
                     RRMLSDGVDLYTARLQEFGIEPSWTPPTPRIGEPVGNPAVDRVLKTVSRWLESATKTW
                     GAPERVIIEHVREGFVTEKRAREMDGDMRRRAARNAKLFQEMQEKLNVQGKPSRADLW
                     RYQSVQRQNCQCAYCGSPITFSNSEMDHIVPRAGQGSTNTRENLVAVCHRCNQSKGNT
                     PFAIWAKNTSIEGVSVKEAVERTRHWVTDTGMRSTDFKKFTKAVVERFQRATMDEEID
                     ARSMESVAWMANELRSRVAQHFASHGTTVRVYRGSLTAEARRASGISGKLKFFDGVGK
                     SRLDRRHHAIDAAVIAFTSDYVAETLAVRSNLKQSQAHRQEAPQWREFTGKDAEHRAA
                     WRVWCQKMEKLSALLTEDLRDDRVVVMSNVRLRLGNGSAHKETIGKLSKVKLSSQLSV
                     SDIDKASSEALWCALTREPGFDPKEGLPANPERHIRVNGTHVYAGDNIGLFPVSAGSI
                     ALRGGYAELGSSFHHARVYKITSGKKPAFAMLRVYTIDLLPYRNQDLFSVELKPQTMS
                     MRQAEKKLRDALATGNAEYLGWLVVDDELVVDTSKIATDQVKAVEAELGTIRRWRVDG
                     FFSPSKLRLRPLQMSKEGIKKESAPELSKIIDRPGWLPAVNKLFSDGNVTVVRRDSLG
                     RVRLESTAHLPVTWKVQ*"
     CDS             complement(2608..3120)
                     /label="ORF frame 3"
                     /translation="MQAVQLGVDVIVQIGKECLHFCSLGRIKIVDFGVGQCLHHGVLL
                     QCASCFPPVDQWCDPAVHNTSVSIVCRGSGSCSLSIVGHSSCSDQLPAIDAQNFGDGH
                     PFGLFLWSQIHQVVEHQVLLLHAQNPLLPIHPDSQIADQCSNPVSLKSVAGFQCSVLS
                     WLQRIFPHPA*"
     CDS             complement(3529..4065)
                     /label="ORF frame 3"
                     /translation="MVPSIQPGLPHAIKEFQLSTDPRCPSSLCCQAASVHPDCGSMGC
                     EVLSHAAPQLISHPGDGFHASGVDFLIHCCSLKPLDHSLGELLEVSASHAGISDPMSG
                     PFHGLLHTDPFDRSVFCPNSEGCIALRLVTAMAHGDQILPCVGGSLPCSGHDVVHFTI
                     RKGNWGPAIGTLAVLSLH*"
     misc_feature    complement(5172..5189)
                     /label="Sp6_primer"
                     /translation="MVPSIQPGLPHAIKEFQLSTDPRCPSSLCCQAASVHPDCGSMGC
                     EVLSHAAPQLISHPGDGFHASGVDFLIHCCSLKPLDHSLGELLEVSASHAGISDPMSG
                     PFHGLLHTDPFDRSVFCPNSEGCIALRLVTAMAHGDQILPCVGGSLPCSGHDVVHFTI
                     RKGNWGPAIGTLAVLSLH*"
     misc_feature    5194..5218
                     /label="attB2"
                     /translation="MVPSIQPGLPHAIKEFQLSTDPRCPSSLCCQAASVHPDCGSMGC
                     EVLSHAAPQLISHPGDGFHASGVDFLIHCCSLKPLDHSLGELLEVSASHAGISDPMSG
                     PFHGLLHTDPFDRSVFCPNSEGCIALRLVTAMAHGDQILPCVGGSLPCSGHDVVHFTI
                     RKGNWGPAIGTLAVLSLH*"
     terminator      5230..5696
                     /label="hGH_PA_terminator"
                     /translation="MVPSIQPGLPHAIKEFQLSTDPRCPSSLCCQAASVHPDCGSMGC
                     EVLSHAAPQLISHPGDGFHASGVDFLIHCCSLKPLDHSLGELLEVSASHAGISDPMSG
                     PFHGLLHTDPFDRSVFCPNSEGCIALRLVTAMAHGDQILPCVGGSLPCSGHDVVHFTI
                     RKGNWGPAIGTLAVLSLH*"
     terminator      5702..5737
                     /label="T7_terminator"
                     /translation="MVPSIQPGLPHAIKEFQLSTDPRCPSSLCCQAASVHPDCGSMGC
                     EVLSHAAPQLISHPGDGFHASGVDFLIHCCSLKPLDHSLGELLEVSASHAGISDPMSG
                     PFHGLLHTDPFDRSVFCPNSEGCIALRLVTAMAHGDQILPCVGGSLPCSGHDVVHFTI
                     RKGNWGPAIGTLAVLSLH*"
     rep_origin      5854..6160
                     /label="f1_origin"
                     /translation="MVPSIQPGLPHAIKEFQLSTDPRCPSSLCCQAASVHPDCGSMGC
                     EVLSHAAPQLISHPGDGFHASGVDFLIHCCSLKPLDHSLGELLEVSASHAGISDPMSG
                     PFHGLLHTDPFDRSVFCPNSEGCIALRLVTAMAHGDQILPCVGGSLPCSGHDVVHFTI
                     RKGNWGPAIGTLAVLSLH*"
     misc_feature    complement(6274..6294)
                     /label="pBABE_3_primer"
                     /translation="MVPSIQPGLPHAIKEFQLSTDPRCPSSLCCQAASVHPDCGSMGC
                     EVLSHAAPQLISHPGDGFHASGVDFLIHCCSLKPLDHSLGELLEVSASHAGISDPMSG
                     PFHGLLHTDPFDRSVFCPNSEGCIALRLVTAMAHGDQILPCVGGSLPCSGHDVVHFTI
                     RKGNWGPAIGTLAVLSLH*"
     misc_feature    complement(6280..6495)
                     /label="SV40_enhancer"
                     /translation="MVPSIQPGLPHAIKEFQLSTDPRCPSSLCCQAASVHPDCGSMGC
                     EVLSHAAPQLISHPGDGFHASGVDFLIHCCSLKPLDHSLGELLEVSASHAGISDPMSG
                     PFHGLLHTDPFDRSVFCPNSEGCIALRLVTAMAHGDQILPCVGGSLPCSGHDVVHFTI
                     RKGNWGPAIGTLAVLSLH*"
     promoter        6292..6560
                     /label="SV40_promoter"
                     /translation="MVPSIQPGLPHAIKEFQLSTDPRCPSSLCCQAASVHPDCGSMGC
                     EVLSHAAPQLISHPGDGFHASGVDFLIHCCSLKPLDHSLGELLEVSASHAGISDPMSG
                     PFHGLLHTDPFDRSVFCPNSEGCIALRLVTAMAHGDQILPCVGGSLPCSGHDVVHFTI
                     RKGNWGPAIGTLAVLSLH*"
     rep_origin      6459..6536
                     /label="SV40_origin"
                     /translation="MVPSIQPGLPHAIKEFQLSTDPRCPSSLCCQAASVHPDCGSMGC
                     EVLSHAAPQLISHPGDGFHASGVDFLIHCCSLKPLDHSLGELLEVSASHAGISDPMSG
                     PFHGLLHTDPFDRSVFCPNSEGCIALRLVTAMAHGDQILPCVGGSLPCSGHDVVHFTI
                     RKGNWGPAIGTLAVLSLH*"
     misc_feature    6521..6540
                     /label="SV40pro_F_primer"
                     /translation="MVPSIQPGLPHAIKEFQLSTDPRCPSSLCCQAASVHPDCGSMGC
                     EVLSHAAPQLISHPGDGFHASGVDFLIHCCSLKPLDHSLGELLEVSASHAGISDPMSG
                     PFHGLLHTDPFDRSVFCPNSEGCIALRLVTAMAHGDQILPCVGGSLPCSGHDVVHFTI
                     RKGNWGPAIGTLAVLSLH*"
     CDS             6675..7469
                     /label="ORF frame 3"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            6678..7466
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(6984..7520)
                     /label="ORF frame 1"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      7646..7765
                     /label="SV40_PA_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    7734..7753
                     /label="EBV_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      7956..7991
                     /label="T7_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    complement(8014..8031)
                     /label="Baculovirs_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    8021..8098
                     /label="3_AcPH"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     CDS             complement(8128..8745)
                     /label="ORF frame 3"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     misc_feature    8725..8758
                     /label="loxP"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     gene            complement(9526..10386)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     CDS             complement(9526..10386)
                     /label="ORF frame 3"

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