| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pEarleyGate202； pEarleyGate 202 |
| 质粒类型: | Gateway载体；植物载体；农杆菌双元表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | Gateway |
| 启动子: | 35S |
| 载体大小: | 11776 bp |
| 5' 测序引物及序列: | 35S promoter：CTATCCTTCGCAAGACCCTTC |
| 3' 测序引物及序列: | -- |
| 载体标签: | FLAG (N ter) |
| 载体抗性: | 卡那霉素、氯霉素（仅空载体） |
| 筛选标记: | 草丁膦（磷丝菌素）phosphinothricin、 |
| 克隆菌株: | DB3.1菌株 |
| 宿主细胞（系）: | 植物细胞、农杆菌 |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃低温保存
- 保质期：
三年
- 英文名：
pEarleyGate202
- 库存：
20
- 供应商：
泽叶生物
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pEarleyGate 202 11776 bp ds-DNA circular SYN 26-JUL-2016
DEFINITION Gateway(R)-compatible plant transformation vector with an N-terminal
FLAG tag.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11776)
AUTHORS Earley KW, Haag JR, Pontes O, Opper K, Juehne T, Song K, Pikaard CS.
TITLE Gateway-compatible vectors for plant functional genomics and
proteomics.
JOURNAL Plant J. 2006;45:616-29.
PUBMED 16441352
REFERENCE 2 (bases 1 to 11776)
AUTHORS Pikaard Lab
TITLE Direct Submission
JOURNAL Exported Tuesday, July 26, 2016 from SnapGene Viewer 3.1.4 to Vector
NTI(R) format
http://www.snapgene.com
COMMENT The sequence was corrected by inserting a G at position 7883.
COMMENT VNTDATE|660009600|
COMMENT VNTDBDATE|776390400|
COMMENT VNTNAME|pEarleyGate 202|
COMMENT VNTAUTHORNAME|Pikaard Lab|
FEATURES Location/Qualifiers
misc_feature 1..25
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
terminator 111..363
/label=MAS terminator
/note="mannopine synthase terminator"
CDS complement(373..924)
/codon_start=1
/gene="Streptomyces hygroscopicus bar"
/product="phosphinothricin acetyltransferase草丁膦（磷丝菌素）
乙酰转移酶"
/label=BlpR
/note="confers resistance to bialophos or phosphinothricin"
promoter complement(930..1310)
/label=MAS promoter
/note="mannopine synthase promoter (Velten et al., 1984)"
promoter 2306..2651
/label=35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
misc_feature 2681..2735
/label=TMV Omega
/note="translational enhancer from the tobacco mosaic virus
5'-leader sequence (Gallie et al., 1988)"
CDS 2737..2739
/codon_start=1
/product="start codon"
/label=ATG
CDS 2740..2763
/codon_start=1
/product="FLAG epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
protein_bind 2767..2886
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter 2916..2946
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
CDS 3000..3659
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
CDS 4001..4306
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
protein_bind complement(4347..4471)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
terminator 4547..5254
/label=OCS terminator
/note="octopine synthase terminator"
misc_feature 5498..5522
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 6822..7451
/codon_start=1
/product="stability protein from Pseudomonas plasmid pVS1"
/label=pVS1 StaA
CDS 7880..8953
/codon_start=1
/product="replication protein from Pseudomonas plasmid
pVS1"
/label=pVS1 RepA
/translation="MDGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 9019..9213
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 9557..9697
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(9883..10471)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(10558..11352)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
ORIGIN
1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
1321 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg
1381 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata
1441 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa
1501 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac
1561 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa
1621 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc
1681 cagcagtgat ccagccccaa aagagatctc ctttgccccg gagattacaa tggacgattt
1741 cctctatctt tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac
1801 cactgataat gagaaggtta gcctcttcaa tttcagaaag aatgctgacc cacagatggt
1861 tagagaggcc tacgcagcag gtctcatcaa gacgatctac ccgagtaaca atctccagga
1921 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaattgcat
1981 caagaacaca gagaaagaca tatttctcaa gatcagaagt actattccag tatggacgat
2041 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt
2101 tcctactgaa tctaaggcca tgcatggagt ctaagattca aatcgaggat ctaacagaac
2161 tcgccgtgaa gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga
2221 aaatcttcgt caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata
2281 cagtctcaga agaccaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc
2341 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
2401 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg
2461 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
2521 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
2581 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
2641 tggagaggac acgctcgagt ataagagctc tatttttaca acaattacca acaacaacaa
2701 acaacaaaca acattacaat tacatttaca attaccatgg actacaaaga cgatgacgac
2761 aaaatcacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat
2821 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc
2881 cagtcatatt ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt
2941 ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa
3001 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
3061 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
3121 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
3181 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
3241 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
3301 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
3361 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
3421 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
3481 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
3541 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
3601 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa
3661 cgcgtggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt
3721 gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat
3781 gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca aggcatatat
3841 gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt
3901 gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa
3961 atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac
4021 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
4081 gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc
4141 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga
4201 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga
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pEarleyGate202载体
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