| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pGL4.30 、pGL4.30 [luc2P/NFAT-RE/Hygro] |
| 质粒类型: | 哺乳细胞表达；信号通路报告载体 |
| 高拷贝/低拷贝: | -- |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6097 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素Ampicillin |
| 筛选标记: | 潮霉素Hygromycin |
| 备注: | pGL4.30载体含有荧光素酶报告基因luc2P和NFAT应答元件。 |
| 产品目录号: | ZY8481 |
| 稳定性: | -- |
| 组成型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
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- 2025年07月15日
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- 详细信息
- 技术资料
- 保存条件：
-20℃低温保存
- 保质期：
三年
- 英文名：
pGL4.30
- 库存：
20
- 供应商：
泽叶生物
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
The pGL4.30[luc2P/NFAT-RE/Hygro] Vector(a–f) contains an NFAT response element (NFAT-RE) that
drives the transcription of the luciferase reporter gene luc2P (Photinus pyralis). luc2P is a synthetically-derived luciferase
sequence with humanized codon optimization that is designed for high expression and reduced anomalous transcription. The
luc2P gene contains hPEST, a protein destabilization sequence. The protein encoded by luc2P responds more quickly than
the protein encoded by the luc2 gene upon induction. The vector backbone contains an ampicillin resistance gene to allow
selection in E. coli and a mammalian selectable marker for hygromycin resistance.
载体序列
LOCUS pGL4.30[luc2P/NFAT-RE/Hygro] 6097 bp DNA SYN
DEFINITION pGL4.30[luc2P/NFAT-RE/Hygro]
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..6097
/organism="pGL4.30[luc2P/NFAT-RE/Hygro]"
/mol_type="other DNA"
CDS 228..2003
/label="ORF frame 3"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAVNSHGFPPEVEEQAAGT
LPMSCAQESGMDRHPAACASARINV*"
terminator complement(2049..2168)
/label="SV40_PA_terminator"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAVNSHGFPPEVEEQAAGT
LPMSCAQESGMDRHPAACASARINV*"
misc_feature complement(2063..2082)
/label="EBV_rev_primer"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAVNSHGFPPEVEEQAAGT
LPMSCAQESGMDRHPAACASARINV*"
misc_feature complement(2390..2410)
/label="pBABE_3_primer"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAVNSHGFPPEVEEQAAGT
LPMSCAQESGMDRHPAACASARINV*"
misc_feature complement(2396..2611)
/label="SV40_enhancer"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAVNSHGFPPEVEEQAAGT
LPMSCAQESGMDRHPAACASARINV*"
promoter 2408..2676
/label="SV40_promoter"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAVNSHGFPPEVEEQAAGT
LPMSCAQESGMDRHPAACASARINV*"
rep_origin 2575..2652
/label="SV40_origin"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAVNSHGFPPEVEEQAAGT
LPMSCAQESGMDRHPAACASARINV*"
misc_feature 2637..2656
/label="SV40pro_F_primer"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAVNSHGFPPEVEEQAAGT
LPMSCAQESGMDRHPAACASARINV*"
CDS 2755..3792
/label="ORF frame 1"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKEVGRV*"
terminator 3816..3864
/label="synth_PA_terminator"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKEVGRV*"
rep_origin complement(4176..4795)
/label="pBR322_origin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKEVGRV*"
CDS complement(4979..5839)
/label="ORF frame 1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
terminator 5944..6097
/label="up_PA_terminator"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
terminator 5944..5992
/label="synth_PA_terminator"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 5944..6097
/label="TB"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 6046..6065
/label="RVprimer3_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCGGAGGAAA AACTGTTTCA TACAGAAGGC
61 GTGGAGGAAA AACTGTTTCA TACAGAAGGC GTGGAGGAAA AACTGTTTCA TACAGAAGGC
121 GTAGATCTAG ACTCTAGAGG GTATATAATG GAAGCTCGAA TTCCAGCTTG GCATTCCGGT
181 ACTGTTGGTA AAAAGCTTGG CAATCCGGTA CTGTTGGTAA AGCCACCATG GAAGATGCCA
241 AAAACATTAA GAAGGGCCCA GCGCCATTCT ACCCACTCGA AGACGGGACC GCCGGCGAGC
301 AGCTGCACAA AGCCATGAAG CGCTACGCCC TGGTGCCCGG CACCATCGCC TTTACCGACG
361 CACATATCGA GGTGGACATT ACCTACGCCG AGTACTTCGA GATGAGCGTT CGGCTGGCAG
421 AAGCTATGAA GCGCTATGGG CTGAATACAA ACCATCGGAT CGTGGTGTGC AGCGAGAATA
481 GCTTGCAGTT CTTCATGCCC GTGTTGGGTG CCCTGTTCAT CGGTGTGGCT GTGGCCCCAG
541 CTAACGACAT CTACAACGAG CGCGAGCTGC TGAACAGCAT GGGCATCAGC CAGCCCACCG
601 TCGTATTCGT GAGCAAGAAA GGGCTGCAAA AGATCCTCAA CGTGCAAAAG AAGCTACCGA
661 TCATACAAAA GATCATCATC ATGGATAGCA AGACCGACTA CCAGGGCTTC CAAAGCATGT
721 ACACCTTCGT GACTTCCCAT TTGCCACCCG GCTTCAACGA GTACGACTTC GTGCCCGAGA
781 GCTTCGACCG GGACAAAACC ATCGCCCTGA TCATGAACAG TAGTGGCAGT ACCGGATTGC
841 CCAAGGGCGT AGCCCTACCG CACCGCACCG CTTGTGTCCG ATTCAGTCAT GCCCGCGACC
901 CCATCTTCGG CAACCAGATC ATCCCCGACA CCGCTATCCT CAGCGTGGTG CCATTTCACC
961 ACGGCTTCGG CATGTTCACC ACGCTGGGCT ACTTGATCTG CGGCTTTCGG GTCGTGCTCA
1021 TGTACCGCTT CGAGGAGGAG CTATTCTTGC GCAGCTTGCA AGACTATAAG ATTCAATCTG
1081 CCCTGCTGGT GCCCACACTA TTTAGCTTCT TCGCTAAGAG CACTCTCATC GACAAGTACG
1141 ACCTAAGCAA CTTGCACGAG ATCGCCAGCG GCGGGGCGCC GCTCAGCAAG GAGGTAGGTG
1201 AGGCCGTGGC CAAACGCTTC CACCTACCAG GCATCCGCCA GGGCTACGGC CTGACAGAAA
1261 CAACCAGCGC CATTCTGATC ACCCCCGAAG GGGACGACAA GCCTGGCGCA GTAGGCAAGG
1321 TGGTGCCCTT CTTCGAGGCT AAGGTGGTGG ACTTGGACAC CGGTAAGACA CTGGGTGTGA
1381 ACCAGCGCGG CGAGCTGTGC GTCCGTGGCC CCATGATCAT GAGCGGCTAC GTTAACAACC
1441 CCGAGGCTAC AAACGCTCTC ATCGACAAGG ACGGCTGGCT GCACAGCGGC GACATCGCCT
1501 ACTGGGACGA GGACGAGCAC TTCTTCATCG TGGACCGGCT GAAGAGCCTG ATCAAATACA
1561 AGGGCTACCA GGTAGCCCCA GCCGAACTGG AGAGCATCCT GCTGCAACAC CCCAACATCT
1621 TCGACGCCGG GGTCGCCGGC CTGCCCGACG ACGATGCCGG CGAGCTGCCC GCCGCAGTCG
1681 TCGTGCTGGA ACACGGTAAA ACCATGACCG AGAAGGAGAT CGTGGACTAT GTGGCCAGCC
1741 AGGTTACAAC CGCCAAGAAG CTGCGCGGTG GTGTTGTGTT CGTGGACGAG GTGCCTAAAG
1801 GACTGACCGG CAAGTTGGAC GCCCGCAAGA TCCGCGAGAT TCTCATTAAG GCCAAGAAGG
1861 GCGGCAAGAT CGCCGTGAAT TCTCACGGCT TCCCTCCCGA GGTGGAGGAG CAGGCCGCCG
1921 GCACCCTGCC CATGAGCTGC GCCCAGGAGA GCGGCATGGA TAGACACCCT GCTGCTTGCG
1981 CCAGCGCCAG GATCAACGTC TAAGGCCGCG ACTCTAGAGT CGGGGCGGCC GGCCGCTTCG
2041 AGCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA
2101 AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG
2161 CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGAGGT
2221 GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTAAAATCG ATAAGGATCC
2281 GTTTGCGTAT TGGGCGCTCT TCCGCTGATC TGCGCAGCAC CATGGCCTGA AATAACCTCT
2341 GAAAGAGGAA CTTGGTTAGC TACCTTCTGA GGCGGAAAGA ACCAGCTGTG GAATGTGTGT
2401 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT
2461 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG
2521 CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG
2581 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT
2641 TATGCAGAGG CCGAGGCCGC CTCTGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT
2701 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC GATTCTTCTG ACACTAGCGC CACCATGAAG
2761 AAGCCCGAAC TCACCGCTAC CAGCGTTGAA AAATTTCTCA TCGAGAAGTT CGACAGTGTG
2821 AGCGACCTGA TGCAGTTGTC GGAGGGCGAA GAGAGCCGAG CCTTCAGCTT CGATGTCGGC
2881 GGACGCGGCT ATGTACTGCG GGTGAATAGC TGCGCTGATG GCTTCTACAA AGACCGCTAC
2941 GTGTACCGCC ACTTCGCCAG CGCTGCACTA CCCATCCCCG AAGTGTTGGA CATCGGCGAG
3001 TTCAGCGAGA GCCTGACATA CTGCATCAGT AGACGCGCCC AAGGCGTTAC TCTCCAAGAC
3061 CTCCCCGAAA CAGAGCTGCC TGCTGTGTTA CAGCCTGTCG CCGAAGCTAT GGATGCTATT
3121 GCCGCCGCCG ACCTCAGTCA AACCAGCGGC TTCGGCCCAT TCGGGCCCCA AGGCATCGGC
3181 CAGTACACAA CCTGGCGGGA TTTCATTTGC GCCATTGCTG ATCCCCATGT CTACCACTGG
3241 CAGACCGTGA TGGACGACAC CGTGTCCGCC AGCGTAGCTC AAGCCCTGGA CGAACTGATG
3301 CTGTGGGCCG AAGACTGTCC CGAGGTGCGC CACCTCGTCC ATGCCGACTT CGGCAGCAAC
3361 AACGTCCTGA CCGACAACGG CCGCATCACC GCCGTAATCG ACTGGTCCGA AGCTATGTTC
3421 GGGGACAGTC AGTACGAGGT GGCCAACATC TTCTTCTGGC GGCCCTGGCT GGCTTGCATG
3481 GAGCAGCAGA CTCGCTACTT CGAGCGCCGG CATCCCGAGC TGGCCGGCAG CCCTCGTCTG
3541 CGAGCCTACA TGCTGCGCAT CGGCCTGGAT CAGCTCTACC AGAGCCTCGT GGACGGCAAC
3601 TTCGACGATG CTGCCTGGGC TCAAGGCCGC TGCGATGCCA TCGTCCGCAG CGGGGCCGGC
3661 ACCGTCGGTC GCACACAAAT CGCTCGCCGG AGCGCAGCCG TATGGACCGA CGGCTGCGTC
3721 GAGGTGCTGG CCGACAGCGG CAACCGCCGG CCCAGTACAC GACCGCGCGC TAAGGAGGTA
3781 GGTCGAGTTT AAACTCTAGA ACCGGTCATG GCCGCAATAA AATATCTTTA TTTTCATTAC
3841 ATCTGTGTGT TGGTTTTTTG TGTGTTCGAA CTAGATGCTG TCGACCGATG CCCTTGAGAG
3901 CCTTCAACCC AGTCAGCTCC TTCCGGTGGG CGCGGGGCAT GACTATCGTC GCCGCACTTA
3961 TGACTGTCTT CTTTATCATG CAACTCGTAG GACAGGTGCC GGCAGCGCTC TTCCGCTTCC
4021 TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA
4081 AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA
4141 AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG
4201 CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG
4261 ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT
4321 CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT
4381 TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC
4441 TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT
4501 GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT
4561 AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC
4621 TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA
4681 AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT
4741 TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT
4801 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
4861 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA
4921 AGTATATATG AGTAAACTTG GTCTGACAGC GGCCGCAAAT GCTAAACCAC TGCAGTGGTT
4981 ACCAGTGCTT GATCAGTGAG GCACCGATCT CAGCGATCTG CCTATTTCGT TCGTCCATAG
5041 TGGCCTGACT CCCCGTCGTG TAGATCACTA CGATTCGTGA GGGCTTACCA TCAGGCCCCA
5101 GCGCAGCAAT GATGCCGCGA GAGCCGCGTT CACCGGCCCC CGATTTGTCA GCAATGAACC
5161 AGCCAGCAGG GAGGGCCGAG CGAAGAAGTG GTCCTGCTAC TTTGTCCGCC TCCATCCAGT
5221 CTATGAGCTG CTGTCGTGAT GCTAGAGTAA GAAGTTCGCC AGTGAGTAGT TTCCGAAGAG
5281 TTGTGGCCAT TGCTACTGGC ATCGTGGTAT CACGCTCGTC GTTCGGTATG GCTTCGTTCA
5341 ACTCTGGTTC CCAGCGGTCA AGCCGGGTCA CATGATCACC CATATTATGA AGAAATGCAG
5401 TCAGCTCCTT AGGGCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCGGTG TTGTCGCTCA
5461 TGGTAATGGC AGCACTACAC AATTCTCTTA CCGTCATGCC ATCCGTAAGA TGCTTTTCCG
5521 TGACCGGCGA GTACTCAACC AAGTCGTTTT GTGAGTAGTG TATACGGCGA CCAAGCTGCT
5581 CTTGCCCGGC GTCTATACGG GACAACACCG CGCCACATAG CAGTACTTTG AAAGTGCTCA
5641 TCATCGGGAA TCGTTCTTCG GGGCGGAAAG ACTCAAGGAT CTTGCCGCTA TTGAGATCCA
5701 GTTCGATATA GCCCACTCTT GCACCCAGTT GATCTTCAGC ATCTTTTACT TTCACCAGCG
5761 TTTCGGGGTG TGCAAAAACA GGCAAGCAAA ATGCCGCAAA GAAGGGAATG AGTGCGACAC
5821 GAAAATGTTG GATGCTCATA CTCGTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT
5881 ACTAGTACGT CTCTCAAGGA TAAGTAAGTA ATATTAAGGT ACGGGAGGTA TTGGACAGGC
5941 CGCAATAAAA TATCTTTATT TTCATTACAT CTGTGTGTTG GTTTTTTGTG TGAATCGATA
6001 GTACTAACAT ACGCTCTCCA TCAAAACAAA ACGAAACAAA ACAAACTAGC AAAATAGGCT
6061 GTCCCCAGTG CAAGTGCAGG TGCCAGAACA TTTCTCT
//
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pGL4.30
¥1250
