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Phospho-P53 (Ser20) Rabbit Pol

yclonal Antibody抗体,orb5571,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb5571
  • 英国
  • 2025年12月03日
  • FC, WB
  • Rabbit
  • Human, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      Phospho-P53 (Ser20) Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      Phospho-P53 (Ser20) Rabbit Polyclonal Antibody

    • 靶点

      TP53

    • 浓度

      1mg/ml

    • 应用范围

      FC, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb5571

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated Synthesised phosphopeptide derived from human P53 around the phosphorylation site of Ser20 TF(p-S)DL

    • 规格

      50 ul

    产品描述:Phospho-P53 (Ser20) Rabbit Polyclonal Antibody

    别名:p53(phospho-Ser20); P53(phospho S20); P53(phospho Ser20); p-P53(Ser20); p-P53(S20); P53_HUMAN; Cellular tumor antigen p53; TP53; Antigen NY-CO-13;Phosphoprotein p53; Tumor suppressor p53; tumor protein p53; BCC7; LFS1; BMFS5; TRP53; P 53; P-53; wt-p53; Widespread p53; Wtp53; Wt p53;

    免疫原:KLH conjugated Synthesised phosphopeptide derived from human P53 around the phosphorylation site of Ser20 TF(p-S)DL

    预测反应性:Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rabbit, Sheep

    分子量:43 kDa

    应用稀释比例:WB=1:500-2000, Flow-Cyt=3μg /test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:P04637

    Note:For research use only.

    产品细节图片1
    Blank control (blue line): Hela (fixed with 70% ethanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-phospho-P53 (Ser20) antibody (orb5571), dilution: 5 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

    产品细节图片2
    Sample: HepG2 Cell Lysate at 30 ug, A549 Cell Lysate at 30 ug, Primary: Anti-phospho-P53 (Ser20) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kD, Observed band size: 48 kD.

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    图标文献和实验
    相关实验
    • Polyclonal Antibody Production

      to the pre-bleed, but get at least 20 mL per rabbit, with 40 mL being the upper limit for an adult rabbit (any more and they get anemic). Monitoring of Titer The quality of the antibodies in serum (humoral immune response) of the bleeds

    • Polyclonal Antibody Production

        Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation.     Rabbit Immunization Initial Preparation Your antigen

    • Polyclonal Antibody Production 简单快捷制备多抗方法,可用于western、ELISA和免疫/b

      the process for the other rabbits. Injections will be done every 4-6 weeks, with bleeds 7-10 days after each injection. Bleeds are performed identically to the pre-bleed, but obtain at least 20 mL per rabbit, with 40 mL being the upper limit for an adult

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