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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
GP73 Antibody抗体
- 抗体英文名:
GP73 Antibody
- 应用范围:
IF, IHC, WB
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat
- 保质期:
6-12个月
- 抗原来源:
详询
- 目录编号:
orb341264
- 级别:
科研级
- 库存:
88
- 供应商:
biorbyt
- 标记物:
Unconjugated
- 克隆性:
Polyclonal
- 形态:
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
- 免疫原:
Recombinant fusion protein of human GP73
- 规格:
30 ul
产品描述:The GP73 Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human GP73 .Purification: The antibody was purified by immunogen affinity chromatography.
别名:C9orf155; GOLPH2; Golgi membrane protein 1; Golgi membrane protein GP73; Golgi phosphoprotein 2
免疫原:Recombinant fusion protein of human GP73
应用稀释比例:WB: 1:500-1:2000, IHC-P: 1:50-1:200, IF/ICC: 1:50-1:100
纯化:The antibody was purified by immunogen affinity chromatography.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Entrez:51280
UniProt ID:Q8NBJ4
Note:For research use only.



别名:C9orf155; GOLPH2; Golgi membrane protein 1; Golgi membrane protein GP73; Golgi phosphoprotein 2
免疫原:Recombinant fusion protein of human GP73
应用稀释比例:WB: 1:500-1:2000, IHC-P: 1:50-1:200, IF/ICC: 1:50-1:100
纯化:The antibody was purified by immunogen affinity chromatography.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Entrez:51280
UniProt ID:Q8NBJ4
Note:For research use only.

Western blot analysis of GP73 expression in SKOV3 (A), SHSY5Y (B) whole cell lysates. (Predicted band size: 44; 45 kD; Observed band size: 75 kD)

Immunohistochemical analysis of GP73 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of GP73 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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GP73 Antibody抗体,orb341264,biorbyt
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