CD43/SPN Rabbit Polyclonal Ant

ibody 抗体，orb196265，Biorbyt

价格￥5499

品牌Biorbyt
货号orb196265
产地英国
更新日期2026年07月22日

应用范围FC, ICC, IF, IHC, IHC-Fr, WB
宿主Rabbit
适应物种Human, Mouse, Rat

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抗体名：
CD43/SPN Rabbit Polyclonal Antibody 抗体
抗体英文名：
CD43/SPN Rabbit Polyclonal Antibody
靶点：
Leukosialin
浓度：
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
应用范围：
FC, ICC, IF, IHC, IHC-Fr, WB
宿主：
Rabbit
适应物种：
Human, Mouse, Rat
保质期：
12 months from date of receipt.
抗原来源：
详询
目录编号：
orb196265
级别：
科研
库存：
99
供应商：
Biorbyt
标记物：
Unconjugated
克隆性：
Polyclonal
保存条件：
详询
形态：
Lyophilized
亚型：
Rabbit IgG
免疫原：
详询
规格：
100 ug

类别: Antibodies

产品子类型: Primary Antibody

描述: CD43/SPN Rabbit Polyclonal Antibody

别名: Leukosialin; Galactoglycoprotein; GALGP; Leukocyte sialoglycoprotein; Sialophorin; CD43; SPN; CD43

应用稀释比例: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Immunocytochemistry, 0.5-1μg/ml, Human, - Immunofluorescence, 2μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human

免疫原: E.coli-derived human CD43 recombinant protein (Position: A272-P400). Human CD43 shares 72% and 73% amino acid (aa) sequences identity with mouse and rat CD43, respectively.

交叉反应: No cross-reactivity with other proteins

分子量: 100-120 kDa

防腐剂: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

纯化方法: Immunogen affinity purified.

复溶/重构: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

特异性: No cross reactivity with other proteins.

研究领域: Cell Biology, Disease Biomarkers, Immunology & Inflammation, Stem Cell & Developmental Biology

UniProt ID: P16150

保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

备注: For research use only.

Flow Cytometry analysis of BRL cells using anti-CD43 antibody. Overlay histogram showing BRL cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% Fixative and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD43 Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Flow Cytometry analysis of HL-60 cells using anti-CD43 antibody. Overlay histogram showing HL-60 cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% Fixative and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD43 Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Flow Cytometry analysis of P3NSI cells using anti-CD43 antibody. Overlay histogram showing P3NSI cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% Fixative and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD43 Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

IF analysis of CD43 using anti-CD43 antibody. CD43 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/mL rabbit anti-CD43 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IHC analysis of CD43 using anti-CD43 antibody. CD43 was detected in immunocytochemical section of K562 Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 µg/ml rabbit anti-CD43 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of CD43 using anti-CD43 antibody. CD43 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-CD43 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of CD43 using anti-CD43 antibody. CD43 was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-CD43 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of CD43 using anti-CD43 antibody. CD43 was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-CD43 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Western blot analysis of CD43 using anti-CD43 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD43 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD43 at approximately 115 KD. The expected band size for CD43 is at 40 KD.

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