RNA polymerase II CTD repeat Y
SPTSPS (phospho S5) Rabbit Polyclonal Antibody抗体，orb100549，biorbyt- ￥3120
- biorbyt
- orb100549
- 英国
- 2025年12月03日
- FC, IF, IHC-Fr, IHC-P
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
RNA polymerase II CTD repeat YSPTSPS (phospho S5) Rabbit Polyclonal Antibody抗体
- 抗体英文名：
RNA polymerase II CTD repeat YSPTSPS (phospho S5) Rabbit Polyclonal Antibody
- 靶点：
POLR2A
- 浓度：
1mg/ml
- 应用范围：
FC, IF, IHC-Fr, IHC-P
- 宿主：
Rabbit
- 适应物种：
Human
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb100549
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated Synthesised phosphopeptide derived from human RNA polymerase II CTD around the phosphorylation site of Ser5 PT(p-S)PS
- 规格：
50 ul
别名:p-Rpb1 CTD(Ser2/Ser5); DNA directed RNA polymerase II A; p-Rpb1 CTD(Ser2/Ser5); DNA-directed RNA polymerase II largest subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; Polr2a; RNA pol II CTD; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RPB1; RPB1_HUMAN.
免疫原：KLH conjugated Synthesised phosphopeptide derived from human RNA polymerase II CTD around the phosphorylation site of Ser5 PT(p-S)PS
预测反应性:Bovine, Canine, Mouse, Rabbit, Rat
分子量:240 kDa
应用稀释比例：IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P24928
Note:For research use only.
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文献和实验Identifying PhosphoCTD-Associating Proteins
The C-terminal repeat domain (CTD) of the largest subunit of RNA polymerase II is hyperphosphorylated during transcription elongation. The phosphoCTD is known to bind to a subset of RNA processing factors
whole-worm extracts. In principle, a protein-specific antibody is used to purify the protein of choice and its associated complex members from worm extract. This may also include RNA molecules associated with other protein components. To identify
Chromatin Immunoprecipitation (ChIP)
The first chromatin immunoprecipitation (ChIP) assay was developed by Gilmour and Lis (1984, 1985, 1986) as a technique for monitoring the association of RNA polymerase II with transcribed and poised genes in Escherichia coli and Drosophila . The use
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