- ￥6110
- biorbyt
- 英国
- orb246821
- 2025年12月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 保质期：
6个月
- 英文名：
Yeast neurotoxin type E protein
- 库存：
详询客服
- 供应商：
biorbyt
- 规格：
20 ug
产品别名：Botulinum neurotoxin type E, BoNT/E, Bontoxilysin-E) [Cleaved into, Botulinum neurotoxin E light chain, LC, EC 3.4.24.69), Botulinum neurotoxin E heavy chain, HC)]
应用笔记：This is His-tag protein
纯度：Greater than 90% as determined by SDS-PAGE.
生物来源：Clostridium butyricum
蛋白序列：PTINSFNYNDPVNNRTILYIKPGGCQQFYKSFNIMKNIWIIPERNVIGTIPQDFLPPTSLKNGDSSYYDPNYLQSDQEKDKFLKIVTKIFNRINDNLSGRILLEELSKANPYLGNDNTPDGDFIINDASAVPIQFSNGSQSILLPNVIIMGAEPDLFETNSSNISLRNNYMPSNHGFGSIAIVTFSPEYSFRFKDNSMNEFIQDPALTLMHELIHSLHGLYGAKGITTKYTITQKQNPLITNIRGTNIEEFLTFGGTDLNIITSAQSNDIYTNLLADYKKIASKLSKVQVSNPLLNPYKDVFEAKYGLDKDASGIYSVNINKFNDIFKKLYSFTEFDLATKFQVKCRQTYIGQYKYFKLSNLLNDSIYNISEGYNINNLKVNFRGQNANLNPRIITPITGRGLVKKIIRFCKNIVSVKGIR
分子量：49.7 kDa
表达区域：2-422aa
标签：N-terminal 6xHis-tagged
防腐剂：If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
来源：Yeast
形态：Liquid or Lyophilized powder
Uniprot ID：P30995
Note：For research use only.
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文献和实验E.Z.N.A.® Yeast Plasmid Mini Spin Protocol
on the yeast strain and type of plasmid. High copy number plasmids generally yield up to 1ug of DNA from 5 ml culture. The ratio of (absorbance260)/(absorbance280) is an indication of nucleic acid purity. A value greater than 1.8 indicates > 90% nucleic acid
Transfer of YAC Clones to New Yeast Hosts
the removal of undesired insert DNA (e.g., neighboring coding sequences or chimeric segments), the introduction of new selectable markers, or the replacement of wild-type DNA with defined mutant alleles. To use existing vector systems for YAC manipulation
Yeast Chromatin Immunoprecipitation (ChIP)
total protein)to a new 1.5 ml tube for IP 2.Include necessary controls (i.e.no Ab control; untagged strain+ Ab) 3.Bring all 1mg aliquots to 200μl with ChIP lysis buffer+protease inhibitors,mix well (thorough mixing is very important) 4.Transfer 2μl
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