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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
- 保质期:
6个月
- 英文名:
Human Cathepsin B Protein, His Tag
- 库存:
详询客服
- 供应商:
biorbyt
- 规格:
50 ug
应用笔记:This protein carries a polyhistidine tag at the C-terminus. The Human Cathepsin B will be further processed into mature form (Leu 80-Ile 339). The protein has a calculated MW of 36.7 kDa (pro-form) and 29.5 kDa (mature-form). The protein migrates as 30-32 kDa and 38-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
反应种属:Human
纯度:95%
分子量:36.7 kDa & 29.5 kDa
表达区域:Arg 18 - Ile 339
内毒素:1.0 EU per μg
标签:C-6×His
防腐剂:50 mM Tris, 150 mM NaCl, pH 8.0
形态:Powder
标记物:Unconjugated
Pubmed:26310922
Uniprot ID:P07858
Note:For research use only.

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文献和实验组织蛋白酶 cathepsin 在各种动物组织的细胞内(特别是溶酶体部分)发现的一类蛋白酶。此名源自希腊语的“消化”,为威尔斯达特( R. Willstā atter, 1929)命名的。来源于同一种类组织细胞的也是由组织蛋白酶 A. B. C. D. E等多种酶组成。可以根据所作用的代表性底物的种类进行分类,但似乎各自还有数种同工酶。组织蛋白酶 A的代表性底物是 N-苄氧基羰基 -L-谷酰胺 -L-苯丙氨酸,最适 pH约 5.5( 3— 6),可能是一种羧肽酶,可为二异丙基磷氟酸
蛋白分离纯化概述(An overview of protein isolation)
the same (Fig. 1 .). Figure 1. A schematic representation of a protein isolation Initially (Fig. 1A) there is a small amount of the desired protein “a” and a large amount of total protein “b”. In the course of the isolation, ìbî
The heterodimeric integral membrane protein flavocytochrome b (Cyt b) is the catalytic core of the phagocyte NADPH oxidase, an enzyme complex that initiates a cascade of reactive oxygen species critical for the elimination of infectious
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