- ￥6110
- biorbyt
- 英国
- orb358493
- 2025年12月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 保质期：
6个月
- 英文名：
Fungi Nuclease P1 protein
- 库存：
详询客服
- 供应商：
biorbyt
- 规格：
20 ug
产品别名：Deoxyribonuclease P1 Endonuclease P1
应用笔记：This is His-tag protein
纯度：Greater than 90% as determined by SDS-PAGE.
生物来源：Penicillium citrinum
蛋白序列：WGALGHATVAYVAQHYVSPEAASWAQGILGSSSSSYLASIASWADEYRLTSAGKWSASLHFIDAEDNPPTNCNVDYERDCGSSGCSISAIANYTQRVSDSSLSSENHAEALRFLVHFIGDMTQPLHDEAYAVGGNKINVTFDGYHDNLHSDWDTYMPQKLIGGHALSDAESWAKTLVQNIESGNYTAQAIGWIKGDNISEPITTATRWASDANALVCTVVMPHGAAALQTGDLYPTYYDSVIDTIELQIAKGGYRLANWINEIHGSEIAK
分子量：31.2 kDa
表达区域：1-270aa
标签：N-terminal 6xHis-tagged
防腐剂：If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
来源：Yeast
形态：Liquid or Lyophilized powder
Uniprot ID：P24289
Note：For research use only.
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文献和实验A practical method for the preparation of total DNA from filamentous fungi
Most methods of DNA preparation from fungi are time-consuming due to the need to first make protoplasts, expensive for chemicals such as cesium chloride, or suitable only for small scale preparations. We have developed a simple method
A practical method for the preparation of total DNA from filamentous fungi
A practical method for the preparation of total DNA from filamentous fungi M.I. Borges, M.O. Azevedo, R. Bonatelli, Jr., M.S.S. Felipe and S. Astolfi-Filho Departamento de Biologia Celular, Universidade de Brasilia, 70910 Brasilia
Modification of the 32P-Postlabeling Method to Detect a Single Adduct Species as a Single Spot
-, and/or oligo-deoxynucleotides, it is further treated with phosphatase and phosphodiesterase prior to TLC. The labeled digest is treated with nuclease P1 (NP1) in method I, and with T4 polynucleotide kinase and NP1 in method II, and then with phosphodiesterase
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