- ￥6890
- biorbyt
- orb348903
- 英国
- 2026年06月01日
- Blocking, FC, IF, IHC, IP
- Rat
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Anti-CD16/CD32 [2.4G2]抗体
- 抗体英文名：
Anti-CD16/CD32 [2.4G2]
- 靶点：
CD16/CD32
- 浓度：
1 mg/ml
- 应用范围：
Blocking, FC, IF, IHC, IP
- 宿主：
Rat
- 适应物种：
Mouse
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb348903
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Monoclonal
- 亚型：
Rat IgG
- 免疫原：
Mouse BALB/c Macrophage J774 Cell Line
- 规格：
0.2 mg
别名:Fcγ III/II Receptor; Low affinity immunoglobulin gamma Fc region receptor III; IgG Fc receptor III; Fc-gamma RIII; FcRIII; CD16; Low affinity immunoglobulin gamma Fc region receptor II; Fc gamma receptor IIB; Fc-gamma RII; Fc-gamma-RIIB; FcRII IgG Fc rec
免疫原：Mouse BALB/c Macrophage J774 Cell Line
克隆性:2.4G2
应用注释：This antibody binds murine Fc Receptors CD16/CD32 and inhibits binding of antibodies to these. It can therefore be used as an Fc-blocking reagent, to reduce non-specific staining of cells by antibodies binding via their Fc-region
纯度:Purified
防腐剂:PBS with 0.02% Proclin 300.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Pubmed:90108
UniProt ID:P08101, P08508
Note:For research use only.
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文献和实验Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD16
Immunofluorescent Staining of Mouse and Rat Leukocytes
-up, please see description in "Procedure for Setting Compensation for Multi-Color Flow Cytometric Analysis". For reducing FcgII/IIIR-mediated antibody binding (or binding of SAv-PE or SAv-Cy-Chrome) which could contribute to background, the use of anti-mouse CD32/CD16
加入 1 μg/106 细胞 Anti-Mouse CD16/CD32 抗体封闭荧光抗体 Fc 受体引起的非特异性染色。震荡混匀，4℃ 孵育 30 min（或室温避光 15 min）。Wash Buffer 液洗涤，1 500 rpm，离心 5 min，弃上清。4. 用适量体积 Wash Buffer 液重悬每个样本，将各组细胞等细胞数混合后分装到单染管和不染抗体管，作为流式检测时调节电压。5. 单染管中加入相应表面染色抗体，同时在每个样本中加入 1 μL percp CD3/106细胞和 0.5 μ
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